These Fcgrt knock-out mice exhibit low plasma albumin concentrations and are resistant to several experimentally induced autoimmune disorders, and are sensitive to experimentally induced Lyme's disease arthritis.
Read More +Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Fcgrt | Fc receptor, IgG, alpha chain transporter |
The neonatal Fc receptor, which is encoded by Fcgrt bound non-covalently to beta-2 microglobulin, facilitates the uptake of IgG through intestinal epithelial cells, protects IgG and albumin from degradation, and is involved in clearance of amyloid beta peptide from the brain. Mice that are homozygous for Fcgrttm1Dcr, a knockout allele, are viable and fertile. No Fcgrt gene product (mRNA or protein) is detected by quantitative PCR analysis of liver cDNA or Western blot analysis of small intestine from homozygotes. Homozygotes exhibit plasma albumin concentrations approximately 45% of levels observed in wildtype controls, reduced IgG levels and diminished perinatal maternal IgG transport. Fcgrttm1Dcr homozygotes are resistant to experimentally induced, serum-transfer autoimmune arthritis, as well as experimentally induced bullous pemphigoid, pemphigus foliaceus, and pemphigus vulgaris. Homozygotes are more sensitive to experimentally induced Lyme's disease arthritis, with more severe ankle swelling, joint involvement and decreased serum levels of anti Borrelia burgdorferi antibodies. IgG is accumulated in the glomerular basement membranes of aging mutants, and clearance of IgG from the kidneys is impaired.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Allele Name | targeted mutation 1, Derry C Roopenian |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | FcRn- |
Gene Symbol and Name | Fcgrt, Fc receptor, IgG, alpha chain transporter |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 7 |
Molecular Note | Sequence from exon 1 and part of exon2 was replaced with a PGK-neo cassette. Quantitative PCR of liver cDNA indicated the absence of mRNA. Western blot analysis of neonatal intestinal extracts failed to reveal protein product. |
When using the FcRn- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003988 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Fcgrt<tm1Dcr> |
Frozen Mouse Embryo | NOD.129X1-Fcgrt<tm1Dcr>/DvsJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD.129X1-Fcgrt<tm1Dcr>/DvsJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD.129X1-Fcgrt<tm1Dcr>/DvsJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | NOD.129X1-Fcgrt<tm1Dcr>/DvsJ Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.