Specific cre expression in neuronal cells of these mice is controlled by the rat Synapsin I promoter. These mice may be useful when mated to floxed genes of interest in studies of neural development and disorders such as Neurofibromatosis type 1.
Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara
Genetic Background | Generation |
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N?+N4F4
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Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$270.00 Domestic price for female 4-week |
348.51 Domestic price for breeder pair |
These transgenic mice express Cre recombinase under the direction of a synapsin promoter. The transgene integrated into chromosome 6, as detected by targeted locus amplification (TLA). Recombinase activity is detected in neuronal cells by embryonic day 12.5. Of note, transgene expression is active in the male germline (seminiferous tubules) and can result in germline recombination in progeny arising from a hemizygous transgenic male. See additional expression information below.
Although homozygotes are viable, lower than expected Mendelian ratios from hemizygous x hemizygous crosses are produced. Homozygotes are fertile, but litter size and pup size produced from breeding with homozygous animals are small, and most pups from homozygous matings do not survive to wean.
Luo et al. 2020 Neuron 106:37 Table 1 shows germline recombination in offspring (F2) of Cre;floxed double mutant (F1) mice bred to floxed and/or wildtype mice. The authors also note that in general, the frequency of recombination in Cre;floxed double mutant germline cells appears to be considerably higher than in zygotes produced by breeding Cre mice to floxed mice.
This reports that Syn1-cre;floxed double mutant males bred to floxed females produced some offspring with germline deletion of the floxed allele [63%]. As such, for Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding Syn1-cre females to floxed males.
If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry. This same information may also be found searching the MGI Recombinase Activity and MGI Gene Expression + Recombinase Activity Comparison Matrix.
A transgenic construct containing a 4 kb rat synapsin promoter directing expression of Cre recombinase was injected into fertilized B6;CBA mouse eggs. Founder line 671 animals were bred to wildtype C57BL/6NHsd mice. The transgene integrated into chromosome 6, as detected by targeted locus amplification (TLA). The mice were backcrossed to wildtype C57BL/6NHsd mice for at least 5 generations. Upon arrival at The Jackson Laboratory, the strain was bred to C57BL/6J (Stock No. 000664). As of November 2020, the live colony has been backcrossed 4 generations to C57BL/6J.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | neuronal cells by embryonic day 12.5 |
Allele Name | transgene insertion 671, Jamey Marth |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | cresyn1; Syn-cre; Syn-Cretg; SynI-Cre |
Gene Symbol and Name | Tg(Syn1-cre)671Jxm, transgene insertion 671, Jamey Marth |
Gene Synonym(s) | |
Promoter | Syn1, synapsin I, rat |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | neuronal cells by embryonic day 12.5 |
Strain of Origin | (C57BL/6 x CBA)F2 |
Chromosome | 6 |
General Note | Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. |
Molecular Note | This transgene expresses Cre recombinase under the control of a rat synapsin I promoter. Cre recombinase activity is detected in neuronal cells, including brain, spinal cord and DRGs, as early as E12.5, as well as in neurons in adult. Line 671 contains 10 copies of the transgene inserted into chromosome 6 over 1 Mb from the nearest gene (chr6:10,423,318; NCBI37/mm9) with a duplication of unknown size at the insertion site. |
Mutations Made By | Jamey Marth, Burnham Inst at Univ Calif Santa Barbara |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The expected coat color from breeding is black.
Of note, transgene expression is active in the male germline (seminiferous tubules). As such, for Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding Syn1-cre females to floxed males. See Detailed Description for more details.
Although homozygotes are viable, lower than expected Mendelian ratios from hemizygous x hemizygous crosses are produced. Homozygotes are fertile, but litter size and pup size produced from breeding with homozygous animals are small, and most pups from homozygous matings do not survive to wean.
When using the B6.Cg-Tg(Syn1-cre)671Jxm/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #003966 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Syn1-cre)671Jxm |
Frozen Mouse Embryo | B6.Cg-Tg(Syn1-cre)671Jxm/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Syn1-cre)671Jxm/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Syn1-cre)671Jxm/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Syn1-cre)671Jxm/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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