Expression of lacZ in these mice is under the control of the CMV enhancer/chicken actin promoter and is widespread except in liver and lung tissue. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with GFP in tissues expressing cre.
Corrinne Lobe, Sunnybrook & Women's College HSC
Genetic Background | Generation |
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Allele Type |
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Transgenic (Conditional ready (e.g. floxed), Reporter) |
These Z/EG transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being liver and lung tissue. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with enhanced GFP expression in tissues expressing cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess cre excision activity in live animals and cells. Although homozygotes are viable, attempts to breed homozygous mice proved unsuccessful.
The Z/EG transgene was designed with the CMV enhancer/chicken beta-actin promoter, a loxP-flanked Bgeo reporter/SV40 polyadenylation signal segment, and an enhanced GFP gene followed by a rabbit beta-globin polyadenylation sequence. This transgene was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells expressing lacZ were selected and utilized in ES cell/diploid embryo aggregation in conjunction with CD1 outbred embryos resulting in chimeric animals. This strain was maintained by the donating investigator as a hemizygote by crosses to wild-type outbred CD1 mice for at least 3 generations prior to arrival at The Jackson Laboratory. Upon arrival, these mice were bred to C57BL/6J for at least one generation to establish the colony.
Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli |
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Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ is widely expressed throughout embryonic and adult tissues with notable exceptions being liver and lung; when crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with Enhanced Green Fluorescent Protein expression |
Allele Name | transgene insertion 21, Corrinne Lobe |
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Allele Type | Transgenic (Conditional ready (e.g. floxed), Reporter) |
Allele Synonym(s) | [ACTBBgeo/GFP] 21Lbe; pCaggs-lacZ-GFP; Tg(ACTB-Bgeo/GFP)21Lbe; Tg(CAG-Bgeo/GFP)21Lbe; Z/EG; Z/EGLobe21; ZEG |
Gene Symbol and Name | Tg(CAG-Bgeo/GFP)21Lbe, transgene insertion 21, Corrinne Lobe |
Gene Synonym(s) | |
Promoter | ACTB, actin, beta, chicken |
Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ is widely expressed throughout embryonic and adult tissues with notable exceptions being liver and lung; when crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with Enhanced Green Fluorescent Protein expression |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 5 |
General Note | Expression of lacZ is widespread in transgenic mice, with notable exceptions being liver and lung tissue. This expression is observed throughout all embryonic and adult stages. |
Molecular Note | This transgene contains a CMV enhancer/chicken beta actin promoter, a segment consisting of floxed lacZ and neomycin resistance genes/SV40 polyadenylation signal, and an enhanced GFP reporter gene followed by a rabbit beta-globin polyadenylation sequence. Expression of lacZ in transgenic mice is widespread, with notable exceptions being liver and lung tissue. This expression is observed throughout all embryonic and adult stages. This transgene was inserted in the G1 band of Chromosome 5 at approximately 136.35 MB (build 37). |
Mutations Made By | Corrinne Lobe, Sunnybrook & Women's College HSC |
When maintaining a live colony, hemizygous mice are bred with wildtype (noncarrier) mice. Although homozygotes are viable, attempts to breed homozygous mice proved unsuccessful.
When using the Z/EG mouse strain in a publication, please cite the originating article(s) and include JAX stock #003920 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(CAG-Bgeo/GFP)21Lbe |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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