| Allele Name | transgene insertion 21, Corrinne Lobe |
| Allele Type | Transgenic (Conditional ready (e.g. floxed), Reporter) |
| Allele Synonym(s) | [ACTBBgeo/GFP] 21Lbe; pCaggs-lacZ-GFP; Tg(ACTB-Bgeo/GFP)21Lbe; Tg(CAG-Bgeo/GFP)21Lbe; Z/EG; Z/EGLobe21; ZEG |
| Gene Symbol and Name | Tg(CAG-Bgeo/GFP)21Lbe, transgene insertion 21, Corrinne Lobe |
| Gene Synonym(s) | |
| Promoter | ACTB, actin, beta, chicken |
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| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli |
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| Expressed Gene | GFP, Green Fluorescent Protein, |
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| Expressed Gene | lacZ, beta-galactosidase, E. coli |
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| Site of Expression | lacZ is widely expressed throughout embryonic and adult tissues with notable exceptions being liver and lung; when crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with Enhanced Green Fluorescent Protein expression |
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| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
| Chromosome | 5 |
| General Note | Expression of lacZ is widespread in transgenic mice, with notable exceptions being liver and lung tissue. This expression is observed throughout all embryonic and adult stages. |
| Molecular Note | This transgene contains a CMV enhancer/chicken beta actin promoter, a segment consisting of floxed lacZ and neomycin resistance genes/SV40 polyadenylation signal, and an enhanced GFP reporter gene followed by a rabbit beta-globin polyadenylation sequence. Expression of lacZ in transgenic mice is widespread, with notable exceptions being liver and lung tissue. This expression is observed throughout all embryonic and adult stages. This transgene was inserted in the G1 band of Chromosome 5 at approximately 136.35 MB (build 37). |
| Mutations Made By | Corrinne Lobe, Sunnybrook & Women's College HSC |
