STOCK Tg(CAG-Bgeo/GFP)21Lbe/J

Stock number: 003920
Product name: Z/EG
Research areas: Research Tools

Description

Expression of lacZ in these mice is under the control of the CMV enhancer/chicken actin promoter and is widespread except in liver and lung tissue. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with GFP in tissues expressing cre.

Detailed description

These Z/EG transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being liver and lung tissue. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with enhanced GFP expression in tissues expressing cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess cre excision activity in live animals and cells. Although homozygotes are viable, attempts to breed homozygous mice proved unsuccessful.

Development

The Z/EG transgene was designed with the CMV enhancer/chicken beta-actin promoter, a loxP-flanked Bgeo reporter/SV40 polyadenylation signal segment, and an enhanced GFP gene followed by a rabbit beta-globin polyadenylation sequence. This transgene was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells expressing lacZ were selected and utilized in ES cell/diploid embryo aggregation in conjunction with CD1 outbred embryos resulting in chimeric animals. This strain was maintained by the donating investigator as a hemizygote by crosses to wild-type outbred CD1 mice for at least 3 generations prior to arrival at The Jackson Laboratory. Upon arrival, these mice were bred to C57BL/6J for at least one generation to establish the colony.

Allele

Symbol: Tg(CAG-Bgeo/GFP)21Lbe
Name: transgene insertion 21, Corrinne Lobe
MGI accession ID: MGI:3046177
Generation method: Transgenic
Attributes: Conditional ready (e.g. floxed); Reporter
Marker symbol: Tg(CAG-Bgeo/GFP)21Lbe
Marker name: transgene insertion 21, Corrinne Lobe
Chromosome: 5
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: GFP,Green Fluorescent Protein,; Bgeo,fusion of beta-galactosidase and neomycin phosphotransferase genes,E. coli; lacZ,beta-galactosidase,E. coli
Site of expression: lacZ is widely expressed throughout embryonic and adult tissues with notable exceptions being liver and lung; when crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with Enhanced Green Fluorescent Protein expression
Molecular note: This transgene contains a CMV enhancer/chicken beta actin promoter, a segment consisting of floxed lacZ and neomycin resistance genes/SV40 polyadenylation signal, and an enhanced GFP reporter gene followed by a rabbit beta-globin polyadenylation sequence. Expression of lacZ in transgenic mice is widespread, with notable exceptions being liver and lung tissue. This expression is observed throughout all embryonic and adult stages. Upon exposure to cre recombinase to excise the lacZ-polyA cassette, EGFP is expressed in cre dependant manner. This transgene was inserted in the G1 band of Chromosome 5 at approximately 136.35 MB (build 37).
General note: Expression of lacZ is widespread in transgenic mice, with notable exceptions being liver and lung tissue. This expression is observed throughout all embryonic and adult stages.