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NOD.129S2(B6)-Ighmtm1Cgn/Dvs
Stock No: 003903
  • Congenic
  • Targeted Mutation
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  • Overview
  • Details
    • Detailed Description
    • Development
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    • Selected References
  • Genetics
  • Disease/Phenotype
    • Disease Terms
    • Research Areas By Phenotype
    • Mammalian Phenotype Terms by Genotype
    • References
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    • Genotyping Protocols
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  • Related Strains

Overview

NOD mice homozygous for the Ighm tm1Cgn allele do not express membrane-bound IgM, lack mature B cells, are free of insulitis, and do not develop IDDM.

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Genetic overview

Genetic Background Generation
N11F16
(2017-10-13 00:00:00)

Ighmtm1Cgn

Allele Type Gene Symbol Gene Name
Targeted (Null/Knockout) Ighm immunoglobulin heavy constant mu
View Genetics

Research Applications

  • Research Tools
  • Diabetes and Obesity Research
  • Immunology, Inflammation and Autoimmunity Research
View All Research Applications

Base Price

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Details

Detailed Description

Ighm deficient mice lack mature B cells. NOD mice homozygous for the Ighmtm1Cgn allele are virtually free of insulitis: at most, presence of only perivascular/periductal leukocytes is detected. No intra-islet infiltrates are found. Results indicate that insulin dependent diabetes mellitus (IDDM) is not delayed, but prevented because of a lack of B cell autoreactive T cell responses. Therefore, the elimination of B lymphocytes by congenic transfer of the Ighmtm1Cgn mutation is sufficient to block IDDM development in an otherwise susceptible NOD mouse strain. (Kitamura 1991, Serreze 1996)

Development

The Ighmtm1Cgn allele was generated in 129S2-derived D1 ES Cells and the founder was immediately bred to C57BL/6 in the laboratory of Dr. Klaus Rajewsky. Subsequent to the importation of this allele into The Jackson Laboratory, Dr. David Serreze backcrossed this allele onto his NOD/ShiLtDvs inbred strain for 10 generations, maintained it by sibling intercrossing homozygotes for several generations and did an additional backcross to NOD/ShiLtDvs in approximately 2015 before again intercrossing to homozygosity and maintaining by sibling intercrossing.

Control Suggestions

  • Multiple; Inquire

Additional Information

  • Considerations for Choosing Controls

Selected References

  • Rivas EI; Driver JP; Garabatos N; Presa M; Mora C; Rodriguez F; Serreze DV; Stratmann T. 2011. Targeting of a T cell agonist Peptide to lysosomes by DNA vaccination induces tolerance in the nonobese diabetic mouse. J Immunol 186(7):4078-87PubMed: 21346228MGI: J:170837
  • Serreze DV; Chapman HD; Varnum DS; Hanson MS; Reifsnyder PC; Richard SD; Fleming SA; Leiter EH; Shultz LD. 1996. B lymphocytes are essential for the initiation of T cell-mediated autoimmune diabetes: analysis of a new speed congenic stock of NOD.Ig mu null mice. J Exp Med 184(5):2049-53PubMed: 8920894MGI: J:37287
  • Silveira PA; Chapman HD; Stolp J; Johnson E; Cox SL; Hunter K; Wicker LS; Serreze DV. 2006. Genes within the Idd5 and Idd9/11 Diabetes Susceptibility Loci Affect the Pathogenic Activity of B Cells in Nonobese Diabetic Mice. J Immunol 177(10):7033-41PubMed: 17082619MGI: J:114754
  • Stolp J; Chen YG; Cox SL; Henck V; Zhang W; Tsaih SW; Chapman H; Stearns T; Serreze DV; Silveira PA. 2012. Subcongenic analyses reveal complex interactions between distal chromosome 4 genes controlling diabetogenic B cells and CD4 T cells in nonobese diabetic mice. J Immunol 189(3):1406-17PubMed: 22732593MGI: J:189784
View All References

Genetics

Ighmtm1Cgn

Allele Symbol: Ighmtm1Cgn

Allele Name targeted mutation 1, University of Cologne
Allele Type Targeted (Null/Knockout)
Allele Synonym(s) B cell-; BCKO; BCR-; BKO; Cmu -; Ig- muMT; Ig-; IgH-; IgHmuMT; Igh-6-; Igh-6null; Igh-6tm1Cgn; Igh-6tm1CgnmuMT0; Ighm-; Igmunull; muIgKO; mum-; muMT; muMt-; mu-MT-
Gene Symbol and Name Ighm, immunoglobulin heavy constant mu
Gene Synonym(s)
Strain of Origin 129S2/SvPas
Chromosome 12
Molecular Note A neomycin resistance cassette disrupted one of the membrane exons of the gene encoding immunoglobulin heavy chain of the class mu (IgM).
Mutations Made By

Daisuke Kitamura, University of Cologne

Disease/Phenotype

Disease Terms

No similarity to the expected human disease phenotype was found. One or more human genes are associated with this human disease. The mouse genotype may involve mutations to orthologs of one or more of these genes, but the phenotype did not resemble the disease.

  • type 1 diabetes mellitus

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Ighmtm1Cgn related

  • Research Tools
    • Cancer Research
      • B cell deficiency
    • Immunology, Inflammation and Autoimmunity Research
      • B cell deficiency
  • Immunology, Inflammation and Autoimmunity Research
    • Immunodeficiency
      • B cell deficiency
  • Diabetes and Obesity Research
    • Type 1 Diabetes (IDDM) Analysis Strains
      • NOD Congenics with Mutations Affecting Immunocompetence
  • Immunology, Inflammation and Autoimmunity Research
    • Autoimmunity
      • B cell deficiency
      • Type 1 Diabetes
    • Immunodeficiency
      • B cell deficiency
  • Research Tools
    • Diabetes and Obesity Research

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Ighmtm1Cgn/Ighmtm1Cgn
NOD.129S2-Ighm/DvsJ

cellular phenotype

  • abnormal T cell proliferation
    • T cells fail to proliferate when splenocytes are cultured in vitro with the diabetes autoantigen GAD
    • (MGI Ref ID J:80859)

hematopoietic system phenotype

  • abnormal T cell proliferation
    • T cells fail to proliferate when splenocytes are cultured in vitro with the diabetes autoantigen GAD
    • (MGI Ref ID J:80859)
  • abnormal spleen B cell follicle morphology
    • follicles do not develop in the spleen
    • (MGI Ref ID J:80859)
  • absent B cells
    • B cells are absent in the spleen
    • (MGI Ref ID J:80859)
  • abnormal T cell number
    • the proportion, but not the number, of CD 4 and CD8 T cells found in the spleen is increased due to the absence of B cells
    • (MGI Ref ID J:80859)

immune system phenotype

  • abnormal spleen B cell follicle morphology
    • follicles do not develop in the spleen
    • (MGI Ref ID J:80859)
  • abnormal T cell proliferation
    • T cells fail to proliferate when splenocytes are cultured in vitro with the diabetes autoantigen GAD
    • (MGI Ref ID J:80859)
  • abnormal T cell number
    • the proportion, but not the number, of CD 4 and CD8 T cells found in the spleen is increased due to the absence of B cells
    • (MGI Ref ID J:80859)
  • absent B cells
    • B cells are absent in the spleen
    • (MGI Ref ID J:80859)
  • decreased susceptibility to autoimmune diabetes
    • 13.6% of female mice develop diabetes by 21 weeks of age compared to control NOD mice that have an incidence rate of 95.5% at this age
    • (MGI Ref ID J:80859)

Genotype: Ighmtm1Cgn/Ighmtm1Cgn
NOD.129S2-Ighm

immune system phenotype

  • decreased susceptibility to autoimmune diabetes
    • no male or female homozygotes develop diabetes (assessed by glycosuric levels >3) by 20 weeks of age
    • (MGI Ref ID J:37287)

References

  • Rivas EI; Driver JP; Garabatos N; Presa M; Mora C; Rodriguez F; Serreze DV; Stratmann T. 2011. Targeting of a T cell agonist Peptide to lysosomes by DNA vaccination induces tolerance in the nonobese diabetic mouse. J Immunol 186(7):4078-87PubMed: 21346228MGI: J:170837
  • Serreze DV; Chapman HD; Varnum DS; Hanson MS; Reifsnyder PC; Richard SD; Fleming SA; Leiter EH; Shultz LD. 1996. B lymphocytes are essential for the initiation of T cell-mediated autoimmune diabetes: analysis of a new speed congenic stock of NOD.Ig mu null mice. J Exp Med 184(5):2049-53PubMed: 8920894MGI: J:37287
  • Silveira PA; Chapman HD; Stolp J; Johnson E; Cox SL; Hunter K; Wicker LS; Serreze DV. 2006. Genes within the Idd5 and Idd9/11 Diabetes Susceptibility Loci Affect the Pathogenic Activity of B Cells in Nonobese Diabetic Mice. J Immunol 177(10):7033-41PubMed: 17082619MGI: J:114754
  • Stolp J; Chen YG; Cox SL; Henck V; Zhang W; Tsaih SW; Chapman H; Stearns T; Serreze DV; Silveira PA. 2012. Subcongenic analyses reveal complex interactions between distal chromosome 4 genes controlling diabetogenic B cells and CD4 T cells in nonobese diabetic mice. J Immunol 189(3):1406-17PubMed: 22732593MGI: J:189784

Additional - Ighmtm1Cgn related

Technical Support

CONTACT TECHNICAL SUPPORT
  • Genotyping Protocols

    • Standard PCR:Ighm
    • Standard PCR:Ighm
    • Genotyping resources and troubleshooting
  • Appearance

    • albino, pink eyed
      Related Genotype: A/A Tyrc/Tyrc
  • Citation

    When using the NOD.129S2(B6)-Ighmtm1Cgn/Dvs mouse strain in a publication, please cite the originating article(s) and include JAX stock #003903 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

B5 (Low)

Pricing & Availability

Availability Varies
  • Domestic
  • International

Live Mouse

Age Genotype Price
weeks

Cryorecovery - Pricing

Service/Product Description Price
Homozygous for Igh-6<tm1Cgn>, 1 pair minimum

Related Products and Services

Frozen Mouse EmbryoNOD.129S2(B6)-Ighm<tm1Cgn>/Dvs Frozen Embryos $2595.00
Frozen Mouse EmbryoNOD.129S2(B6)-Ighm<tm1Cgn>/Dvs Frozen Embryos $2595.00
Frozen Mouse EmbryoNOD.129S2(B6)-Ighm<tm1Cgn>/Dvs Frozen Embryos $3373.50
Frozen Mouse EmbryoNOD.129S2(B6)-Ighm<tm1Cgn>/Dvs Frozen Embryos $3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Use of MICE by companies or for-profit entities requires a license prior to shipping.

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Email: TechTran@jax.org

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