NOD.129S2(B6)-Ighm^tm1Cgn/Dvs

Stock number: 003903
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

NOD mice homozygous for the Ighm ^tm1Cgn allele do not express membrane-bound IgM, lack mature B cells, are free of insulitis, and do not develop IDDM.

Detailed description

Ighm deficient mice lack mature B cells. NOD mice homozygous for the Ighm^tm1Cgn allele are virtually free of insulitis: at most, presence of only perivascular/periductal leukocytes is detected. No intra-islet infiltrates are found. Results indicate that insulin dependent diabetes mellitus (IDDM) is not delayed, but prevented because of a lack of B cell autoreactive T cell responses. Therefore, the elimination of B lymphocytes by congenic transfer of the Ighm^tm1Cgn mutation is sufficient to block IDDM development in an otherwise susceptible NOD mouse strain. (Kitamura 1991, Serreze 1996)

Development

The Ighm^tm1Cgn allele was generated in 129S2-derived D1 ES Cells and the founder was immediately bred to C57BL/6 in the laboratory of Dr. Klaus Rajewsky. Subsequent to the importation of this allele into The Jackson Laboratory, Dr. David Serreze backcrossed this allele onto his NOD/ShiLtDvs inbred strain for 10 generations, maintained it by sibling intercrossing homozygotes for several generations and did an additional backcross to NOD/ShiLtDvs in approximately 2015 before again intercrossing to homozygosity and maintaining by sibling intercrossing.

Allele

Symbol: Ighm^tm1Cgn
Name: targeted mutation 1, University of Cologne
MGI accession ID: MGI:1857187
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ighm
Marker name: immunoglobulin heavy constant mu
Chromosome: 12
Strain of origin: 129S2/SvPas
Molecular note: A neomycin resistance cassette disrupted one of the membrane exons of the gene encoding immunoglobulin heavy chain of the class mu (IgM).