B6;129-Irs3^tm1Lhd/J

Stock number: 003900
Product name: IRS-3 KO
Research areas: Diabetes and Obesity Research

Description

These Irs3 knock-out mice exhibit a reduction in fasting plasma insulin level.

Detailed description

Mice that are homozygous null for the Irs3 gene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The inactivation of Irs3 appears not to perturb glucose homeostasis with the possible exception of young (9-13 weeks, postnatal) homozygous males. These mice exhibit a fasting plasma insulin level 60% that of wildtype controls. The difference is not observed in female or older (6 months) homozygous null mice.

Development

A targeting vector containing a herpes simplex virus thymidine kinase gene and a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter was used to disrupt the Irs3 coding region sequence. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male was bred to C57BL/6 females. Offspring animals were bred to homozygosity. This strain arose on a B6;129 background and is maintained on the same.

Allele

Symbol: Irs3^tm1Lhd
Name: targeted mutation 1, Gustav E Lienhard
MGI accession ID: MGI:2149647
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Irs3
Marker name: insulin receptor substrate 3
Chromosome: 5
Strain of origin: 129S4/SvJae
Molecular note: A neomycin resistance cassette disrupted the coding region of the gene.