Mice homozygous for both curly bare (cub) and the recessive, C57BL/6 allele at the modifier of curly bare locus (mcub) appear hairless or "fuzzy." cub homozygotes that are either heterozygous or homozygous for the dominant, V/Le-derived Mcub allele have wavy fur. cub/+ mice of any Mcub genotype have normal fur. Stock no. 003826 is maintained segregating for cub and is homozygous for mcub, so the cub/cub mice of this strain are hairless.
The cub mutation, which arose in STOCK a/a us/us (stock no. 000319), was identified by the hairless or "fuzzy" appearance of cub/cub mice. A cross was performed to the V/Le linkage testing stock for an allele test with fz, (fuzzy), which was negative. Some of the F2 segregants of this cross displayed wavy fur. This proved to be due to an interaction of cub/cub with a dominant modifier of cub, called Mcub, which was present in the V/Le stock. In the absence of Mcub, cub/cub mice are bare, but in the presence of the modifier they have wavy coats. The 003628 colony is homozygous for both cub and Mcub; 003826 is maintained segregating for cub and lacks Mcub. Embryos (also designated 003628) have been cryopreserved from a cross of C57BL/6J female mice and males of 003628; pups recovered from these embryos will be heterozygous at both loci and so will have normal fur. Interbreeding of these animals can regenerate both 003628 and 003826.
|Allele Name||curly bare|
|Allele Type||Spontaneous (Not Applicable)|
|Gene Symbol and Name||Rhbdf2, rhomboid 5 homolog 2|
|Strain of Origin||STOCK a us|
|Molecular Note||A spontaneous mutation resulted in a 12,681 bp deletion removing exons 2-6. An aberrant transcript containing exon 1 spliced to exon 7 was detected in homozygous mice. Noncomplementation with Rhbdf2tm1a(KOMP)Wtsi indicates that this is a gain of function mutation.|
When using the curly bare mouse strain in a publication, please cite the originating article(s) and include JAX stock #003826 in your Materials and Methods section.