These Ttpa knock-out mice exhibit reduction in alpha-tocopherol rendering female mice infertile.
Robert V Farese, Jr., Harvard University School of Public Health
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Ttpa | tocopherol (alpha) transfer protein |
Mice that are homozygous null for the Ttpa gene are viable, normal in size and do not display any gross physical abnormalities. The Ttpa protein product is required for the maintenance of proper alpha-tocopherol levels, the major form of vitamin E in plasma and tissues. The absence of Ttpa protein product in homozygous-null animals results in a corresponding 95% reduction in alpha-tocopherol. Low levels of alpha-tocopherol render female mice infertile, a condition that can be addressed with vitamin E supplements. Male fertility is unimpaired. These mice provide a viable model for studying vitamin E deficiency.
A targeting vector containing thymidine kinase, lacZ and neomycin resistance genes was used to disrupt the Ttpa exons 1 and 2. The construct was transfected into 129S4/SvJae-derived RF8 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were backcrossed to C57BL/6J mice.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression |
Allele Name | targeted mutation 1, Bob Farese |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | alpha-TTP-KO; Ttpa- |
Gene Symbol and Name | Ttpa, tocopherol (alpha) transfer protein |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | 129S4/SvJae |
Chromosome | 4 |
Molecular Note | A lacZ-neomycin resistance cassette replaced part of the 5' untranslated region and exon 1 including the translational start codon. Immunoblot analysis of liver did not detect wild-type protein in homozygous mutant mice. |
Mutations Made By | Robert Farese, Jr., Harvard University School of Public Health |
This strain originated on a B6;129S background and has been backcrossed to C57BL/6J for ten generations before being made homozygous. Homozygous females are infertile due to a vitamin E deficiency. Males have no fertility problems. The donating investigator maintains these mice by crossing homozygous males with heterozygous females.
When using the Ttpa- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003823 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Ttpa<tm1Far> |
Frozen Mouse Embryo | B6.129S4-Ttpa<tm1Far>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Ttpa<tm1Far>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Ttpa<tm1Far>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129S4-Ttpa<tm1Far>/J Frozen Embryos | $3373.50 |
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