B6;129S-Fcgr2btm1Ttk Clcn1adr-mto5J mice homozygous for Clcn1adr-mto5J are affected by a neuromuscular disorder that manifests by weaning as an apparent weakness or stiffness in the rear legs, which is particularly noticeable when the mice are picked up and put down again (as when being transferred to a new cage). The phenotype may disappear as the mice get older. Both male and female homozygotes have normal lifespans and are fertile.
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Fcgr2b | Fc receptor, IgG, low affinity IIb |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Clcn1 | chloride channel, voltage-sensitive 1 |
B6;129S-Fcgr2btm1Ttk Clcn1adr-mto5J mice homozygous for Clcn1adr-mto5J are affected by a neuromuscular disorder that manifests by weaning as an apparent weakness or stiffness in the rear legs, which is particularly noticeable when the mice are picked up and put down again (as when being transferred to a new cage). The phenotype may disappear as the mice get older. Both male and female homozygotes have normal lifespans and are fertile.
The Clcn1adr-mto5J mutation occurred spontaneously in the B6;129S-Fcgr2btm1Ttk/J (002848) colony at The Jackson Laboratory in October, 1999. It was shown to be recessive by the lack of a phenotype in F1 progeny of crosses to C57BL/6J, and it was shown to be a remutation at Clcn1 by its failure to complement Clcn1adr-mto2J in an allele test.
Allele Name | targeted mutation 1, Toshiyuki Takai |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | FcgammaRII; FcgammaRII-; FcgammaRII2B-; FcgammaRIIB-; Fcgr2btm1Rav; FcgRIIB129-; FcRIIB-; IIB-; TAC264 |
Gene Symbol and Name | Fcgr2b, Fc receptor, IgG, low affinity IIb |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 1 |
Molecular Note | A 2.3 kb genomic fragment containing the S1 and EC1 exons was replaced by a neomycin resistance cassette. Encoded transcript was undetectable in homozygous mutant mice via Northern blot analysis (data not shown). Western blot analysis of LPS-stimulated B cells derived from homozygous mice confirmed that no protein was made from this allele, and FACS analysis demonstrated that no functional protein was expressed on the cell surface of B cells in homozygous mice. |
Mutations Made By | Dr. Jeffrey Ravetch, Memorial Sloan Kettering Cancer Center |
Allele Name | myotonia 5 Jackson |
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Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | Clcn1, chloride channel, voltage-sensitive 1 |
Gene Synonym(s) | |
Strain of Origin | B6;129S-Fcgr2btm1Ttk |
Chromosome | 6 |
General Note | Mice homozygous for Clcn1adr-mto5J are affected by a neuromuscular disorder that manifests by weaning as an apparent weakness or stiffness in the rear legs, which is particularly noticeable when the mice are picked up and put down again (as when being transferred to a new cage). The phenotype may disappear as the mice get older. Both male and female homozygotes have normal lifespans and are fertile. |
Molecular Note | This mutation occurred spontaneously in a colony at The Jackson Laboratory in October, 1999. It was shown to be a remutation at Clcn1 by its failure to complement Clcn1adr-mto2J in a complementation test. |
When using the B6;129S-Fcgr2btm1Ttk Clcn1adr-mto5J/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #003817 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Fcgr2b<tm1Ttk>, Heterozygous for Clcn1<adr-mto5J> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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