JAX Mouse Strain Datasheet - 003816

WB.CcS3-Spta1^sph-Dem/BrkJ

Stock No:: 003816 |; sph^Dem

Cryo Recovery

Overview

Also Known As: sph^Dem

These mice carry a spontaneous mutation at the Spta1 locus characterized by severe hereditary elliptocytosis (HE).

Genetic overview

Genetic Background	Generation

Spta1^sph-Dem

Allele Type	Gene Symbol	Gene Name
Spontaneous	Spta1	spectrin alpha, erythrocytic 1

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Research Applications

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

These Spta1^sph-Dem mice exhibit severe hereditary elliptocytosis (HE). The mutation is the insertion of an intracisternal A particle element in intron 10 of the erythroid alpha-spectrin gene. This causes exon skipping, the in-frame deletion of 46 amino acids from repeat 5 of alpha-spectrin and alters spectrin dimer/tetramer stability and osmotic fragility. The disease is more severe in homozygous neonates than in alpha-spectrin-deficient mice with hereditary spherocytosis (HS). Thrombosis and infarction are not limited to adults but occur soon after birth. These mice provide a unique model for analyzing spectrin dimer- to-tetramer conversion and identifying factors that influence thrombosis.

Genetics

Spta1^sph-Dem

Allele Symbol: Spta1^sph-Dem

Allele Name	spherocytosis-Demant
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	Spta1, spectrin alpha, erythrocytic 1
Gene Synonym(s)	AF093576; AI451697; EL2; HPP; HS3; SPH3; SPTA; Spna-1; Spna-1; Spna1; Spna1; erythroid; expressed sequence AF093576; expressed sequence AI451697; ha; hemolytic anemia; iasi hereditary jaundice; ihj; ihj; spectrin alpha 1; sph; spherocytosis
Strain of Origin	CcS3/Dem
Chromosome	1
Molecular Note	Sequence analysis of genomic DNA shows this allele is the result of an intracisternal A particle transposon insertion into intron 10 of the erythroid alpha-spectrin gene. This leads to abnormal splicing and an in-frame deletion of 46 amino acids from repeat 5 of alpha spectrin.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

References

Additional - Spta1^sph-Dem related

Peters LL; Barker JE. 2001. Spontaneous and targeted mutations in erthrocyte membrane skeleton genes: mouse models of heredity spherocytosis. In: Hematopoiesis A Developmental Approach. Oxford University Press. MGI: J:88022
Wandersee NJ; Roesch AN; Hamblen NR; de Moes J; van der Valk MA; Bronson RT; Gimm JA; Mohandas N; Demant P; Barker JE. 2001. Defective spectrin integrity and neonatal thrombosis in the first mouse model for severe hereditary elliptocytosis. Blood 97(2):543-50. PubMed: 11154235 MGI: J:66966
Wandersee NJ; Tait JF; Barker JE. 2000. Erythroid phosphatidyl serine exposure is not predictive of thrombotic risk in mice with hemolytic anemia Blood Cells Mol Dis 26(1):75-83. PubMed: 10772878 MGI: J:62355

Technical Support

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Genotyping Protocols
- Genotyping resources and troubleshooting
Citation
When using the sph^Dem mouse strain in a publication, please cite the originating article(s) and include JAX stock #003816 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or Wildtype for Spna1<sph-Dem>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Licensing Information

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Also Known As: sphDem

Genetic overview

Research Applications

Base Price

Detailed Description

Spta1sph-Dem

Allele Symbol: Spta1sph-Dem

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

References

Additional - Spta1sph-Dem related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: sph^Dem

Spta1^sph-Dem

Allele Symbol: Spta1^sph-Dem

Additional - Spta1^sph-Dem related