In 2020, analysis performed by The Jackson Laboratory discovered Stock No. 003811 (Q58-11) does not exhibit human SCA2 RNA expression from the transgene.
Stefan M Pulst, University of Utah
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence, Humanized sequence) |
In 2020, analysis performed by The Jackson Laboratory discovered Stock No. 003811 does not exhibit human SCA2 RNA expression from the transgene. In addition, this strain has transgene copy number variations indicative of two or more insertion sites. Hemizygotes show 5-8 transgene copies (homozygotes 10-16 transgene copies).
The original characterization of these mice is listed below, but may not describe our current colony.
These transgenic mice express the human SCA2 gene under the direction of the mouse Pcp2 promoter. Homozygous mice are viable and fertile. Transcripts and protein product are detected in the cerebellum. Immunohistochemical analysis indicates that the protein product is localized to the cytoplasm. Progressive functional deficits are evident as early as eight weeks of age as measured by hind leg clasping and stride length. Also noted is a dramatic loss of Purkinje cell arbor followed by a progressive decrease in Purkinje cell number. By 24-27 weeks, the number of Purkinje cells is reduced by approximately 50%. [Huynh et al. 2000 Nature Genetics 26:44 (PMID:10973246)]
A transgenic construct containing a full length SCA2 cDNA (with a 58 CAG repeat) as well as the mouse Pcp2 (Purkinje cell protein 2) promoter, untranslated and poly(A) sequences was injected into B6D2F1 pronuclei. Founder animals were obtained and crossed with B6D2F1 mice.
In 2020, analysis performed by The Jackson Laboratory discovered Stock No. 003811 has transgene copy number variations indicative of two or more insertion sites. Hemizygotes show 5-8 transgene copies (homozygotes 10-16 transgene copies).
Expressed Gene | SCA2, spinocerebellar ataxia 2 (olivopontocerebellar ataxia 2, autosomal dominant, ataxin 2), human |
---|---|
Site of Expression |
Allele Name | transgene insertion 11, Stefan M Pulst |
---|---|
Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | Q58-11; Tg(Pcp2SCA2)11Plt |
Gene Symbol and Name | Tg(Pcp2-SCA2)11Plt, transgene insertion 11, Stefan M Pulst |
Gene Synonym(s) | |
Promoter | Pcp2, Purkinje cell protein 2, rat |
Expressed Gene | SCA2, spinocerebellar ataxia 2 (olivopontocerebellar ataxia 2, autosomal dominant, ataxin 2), human |
Strain of Origin | (C57BL/6J x DBA/2J)F1 |
Chromosome | UN |
Molecular Note | The transgene contains a full length human spinocerebellar ataxia 2 (SCA2) cDNA (with a 58 CAG repeat) as well as the mouse Pcp2 (Purkinje cell protein 2) promoter, untranslated, and poly(A) sequences. Transcripts and protein product are detected in the cerebellum. |
These mice originated on a B6D2F1 background. They are maintained as hemizygotes on the same background. Coat color expected from breeding is black.
When using the Q58-11 mouse strain in a publication, please cite the originating article(s) and include JAX stock #003811 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non-carrier for Tg(Pcp2-SCA2)11Plt |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.