These MLC-Glut1b mice express a transgene containing the human SLC2A1 gene, and exhibit increased amounts of the SLC2A1 protein product in the sarcolemma of skeletal muscle.
Mike M. Mueckler, Washington University School of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Transgenic mice expressing the human SLC2A1 gene under the direction of the rat Myl2 myosin light chain promoter have increased amounts of the SLC2A1 protein product in the sarcolemma of skeletal muscle. Overexpression impacts glucose homeostasis in that glucose administered orally is disposed from the blood more rapidly in transgenic animals resulting in markedly reduced blood glucose levels when compared to wildtype littermates. Although basal glucose transport is enhanced, transgenic animals exhibit impaired glucose transport in response to insulin. This strain is suitable for applications in diabetes and hypoglycemia research.
A transgenic construct containing the rat Myl2 (myosin light chain 2) promoter, a cDNA sequence encoding human SLC2A1, a segment of human albumin intron 1 and a human GAPD (glyceraldehyde-3-phosphate dehydrogenase) polyadenylation site was injected into pronuclei of fertilized B6SJLF2 oocytes. Founder mice obtained and bred to B6SJLF1 females.
Expressed Gene | SLC2A1, solute carrier family 2 member 1, human |
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Site of Expression |
Allele Name | transgene insertion 2, Mike M Mueckler |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | MLC-Glut1b |
Gene Symbol and Name | Tg(Myl2SLC2A1)2Mue, transgene insertion 2, Mike M Mueckler |
Gene Synonym(s) | |
Promoter | Mylpf, myosin light chain, phosphorylatable, fast skeletal muscle, rat |
Expressed Gene | SLC2A1, solute carrier family 2 member 1, human |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
Molecular Note | The transgene contains the rat Myl2 (myosin light chain 2) promoter, a cDNA sequence encoding human SLC2A1, a segment of human albumin intron 1 and a human GAPD (glyceraldehyde-3-phosphate dehydrogenase) polyadenylation site. |
This strain originated on a B6SJLF2 background and has been maintained by breeding male transgenic animals to B6SJLF1 females. They have not yet been successfully bred as homozygotes.
When using the MLC-Glut1b mouse strain in a publication, please cite the originating article(s) and include JAX stock #003804 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Myl2SLC2A1)2Mue |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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