This knock-in strain expresses Cre recombinase under the control of the endogenous Meox2 promoter with Cre recombinase active in early embryos. These mice can be utilized as a deleter strain for floxed genes and provide an alternative to tetraploid embryo analysis.
Dr. Philippe Soriano, Mount Sinai School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Meox2 | mesenchyme homeobox 2 |
This strain expresses Cre recombinase under the control of the endogenous Meox2 promoter. Expression of Cre recombinase is observed in epiblast-derived tissues as early as embryonic day 5. The insertion creates a null allele for the Meox2 gene. Homozygous mice are viable on this background but exhibit an overall reduction in muscle mass and the absence of specific muscles resulting in abnormal limb posture and reduced motility. This phenotype is variable. As many as 80% of homozygotes are severely affected, fail to thrive and die before weaning. Some homozygotes (10%) exhibit clefting of the secondary palate. These mice can be utilized as a deleter strain for loxP flanked DNA and provide an alternative to tetraploid embryo analysis.
A targeting vector containing a nuclear localization sequence-modified Cre recombinase and neomycin resistance genes, linked to a bovine growth hormone polyadenylation was used to replace the first Meox coding exon and 300 base pairs of the following intron in AK7 embryonic stem (ES) cells. Correctly targeted ES cells were used to make chimeric animals which were backcrossed to C57BL/6 mice.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | epiblast-derived tissues as early as embryonic day 5; all primitive ectoderm by embryonic day 7 (but not endoderm or trophectoderm); null allele for Meox2 gene |
Allele Name | targeted mutation 1, Philippe Soriano |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | Meox2Cre; Meox2CreSor; Meox2-Cre; MORE; More-cre; Mox2 Cre; Mox2Cre; Mox2Cre; Mox2-Cre; MoxCre |
Gene Symbol and Name | Meox2, mesenchyme homeobox 2 |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | epiblast-derived tissues as early as embryonic day 5; all primitive ectoderm by embryonic day 7 (but not endoderm or trophectoderm); null allele for Meox2 gene |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 12 |
Molecular Note | An nls-cre gene was inserted at the initiator ATG of the gene, followed by a neomycin selection cassette. The endogenous regulatory sequences drive expression by E5 in the epiblast and by E7 expression is detectable in all primitive ectoderm but not endoderm or trophectoderm. |
Mutations Made By | Dr. Michelle Tallquist, University of Hawaii |
This strain originated on a B6;129S4/SvJaeSor background and has been backcrossed to C57BL/6 for at least six generations. Expected coat color from breeding:Black
When using the MORE mouse strain in a publication, please cite the originating article(s) and include JAX stock #003755 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildytpe for Meox2<tm1(cre)Sor> |
Frozen Mouse Embryo | B6.129S4-Meox2<tm1(cre)Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Meox2<tm1(cre)Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Meox2<tm1(cre)Sor>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S4-Meox2<tm1(cre)Sor>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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