This knock-in strain expresses Cre recombinase under the control of the endogenous Meox2 promoter with Cre recombinase active in early embryos. These mice can be utilized as a deleter strain for floxed genes and provide an alternative to tetraploid embryo analysis.
Dr. Philippe Soriano, Mount Sinai School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Meox2 | mesenchyme homeobox 2 |
This strain expresses Cre recombinase under the control of the endogenous Meox2 promoter. Expression of Cre recombinase is observed in epiblast-derived tissues as early as embryonic day 5. The insertion creates a null allele for the Meox2 gene. Homozygous mice are viable on this background but exhibit an overall reduction in muscle mass and the absence of specific muscles resulting in abnormal limb posture and reduced motility. This phenotype is variable. As many as 80% of homozygotes are severely affected, fail to thrive and die before weaning. Some homozygotes (10%) exhibit clefting of the secondary palate. These mice can be utilized as a deleter strain for loxP flanked DNA and provide an alternative to tetraploid embryo analysis.
A targeting vector containing a nuclear localization sequence-modified Cre recombinase and neomycin resistance genes, linked to a bovine growth hormone polyadenylation was used to replace the first Meox coding exon and 300 base pairs of the following intron in AK7 embryonic stem (ES) cells. Correctly targeted ES cells were used to make chimeric animals which were backcrossed to C57BL/6 mice.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | epiblast-derived tissues as early as embryonic day 5; all primitive ectoderm by embryonic day 7 (but not endoderm or trophectoderm); null allele for Meox2 gene |
Allele Name | targeted mutation 1, Philippe Soriano |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | Meox2tm1(cre)Sor; targeted mutation 1, Philippe Soriano |
Gene Symbol and Name | Meox2, mesenchyme homeobox 2 |
Gene Synonym(s) | expressed sequence AI528662; AI528662; MOX2; Mox-2; GAX; Mox2; Gax |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | epiblast-derived tissues as early as embryonic day 5; all primitive ectoderm by embryonic day 7 (but not endoderm or trophectoderm); null allele for Meox2 gene |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 12 |
Molecular Note | An nls-cre gene was inserted at the initiator ATG of the gene, followed by a neomycin selection cassette. The endogenous regulatory sequences drive expression by E5 in the epiblast and by E7 expression is detectable in all primitive ectoderm but not endoderm or trophectoderm. |
Mutations Made By | Dr. Michelle Tallquist, University of Hawaii |
This strain originated on a B6;129S4/SvJaeSor background and has been backcrossed to C57BL/6 for at least six generations. Expected coat color from breeding:Black
When using the MORE mouse strain in a publication, please cite the originating article(s) and include JAX stock #003755 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service | Genotype | Price |
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Heterozygous or wildytpe for Meox2<tm1(cre)Sor> |
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
Frozen Mouse Embryo | B6.129S4-Meox2<tm1(cre)Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Meox2<tm1(cre)Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Meox2<tm1(cre)Sor>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S4-Meox2<tm1(cre)Sor>/J Frozen Embryo | $3373.50 |
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