C57BL/10ScNJ

Stock number: 003752
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

This C57BL/10 substrain was inbred at NIH until it was sent back to The Jackson Laboratory in 2000. These mice, carrying a spontaneous deletion of the Tlr4 gene, serve as a common genetic background for histocompatibility congenic and mutant strains.

Detailed description

The breeder pair of C57BL/10ScN mice, obtained from NIH, that were the progenitors of all mice of this strain at The Jackson Laboratory were tested by PCR analysis for the spontaneous Il12rb2 mutation described by Poltorak et al. (J. Immunol. 167:2106-2111, 2001) and found to carry only the wild type Il12rb2 allele. C57BL/10ScN mice have a deletion of the Tlr4 gene that results in absence of both mRNA and protein and thus in defective response to LPS stimulation. Tlr4^lps-del differs from the Tlr4^Lps-d mutation of C3H/HeJ mice, a point mutation that causes an amino acid substitution. The allele for normal LPS response, Tlr4^lps-n, occurs in most other C3H and C57BL/10 substrains and most other mouse strains.

Development

The C57BL/6 and C57BL/10 sublines of the C57BL mouse line diverged sometime prior to 1937. The C57BL/10 subline was passed from Clarence Little to J.P. Scott (lab code Sc) at F26 and from Scott to George Snell (Sn) at F35 around 1947. In 1953, Snell sent mice of this line, C57BL/10ScSn (also known as C57BL/10Sn) to the National Institutes of Health (N) (Vogel et al. 1979), where it was initially designated C57BL/10ScSnN; however, Snell's lab code was dropped early on, and the strain has been called C57BL/10ScN for most of its history at NIH (Hansen et al. 2000). Clarence Reeder (Cr) began maintaining mice of this line at the Frederick Cancer Research Center, Frederick, MD in 1961 (Vogel et al. 1979), and mice distributed from this colony were designated C57BL/10ScNCr (Hansen et al. 2000). The breeding stock at the Cancer Research Center is replenished regularly with C57BL/10ScN from NIHAGR (Hansen et al. 2000), and mice of both colonies are screened annually for 14 biochemical markers and every two years by skin grafting; therefore, C57BL/10ScN, C57BL/10ScNCr and, presumably, C57BL/10ScCr are names for the same subline of C57BL/10.

A pedigreed sibling pair of C57BL/10ScN mice obtained in August, 2000 at N130 from the NIH foundation colony was used to found the colony now maintained at The Jackson Laboratory.

Allele

Symbol: Tlr4^lps-del
Name: defective lipopolysaccharide response, deletion
MGI accession ID: MGI:1860755
Generation method: Spontaneous
Attributes: Null/Knockout
Synonyms: Tlr4^-; TLR4^lps-; TLR4-KO
Marker symbol: Tlr4
Marker name: toll-like receptor 4
Marker synonyms: ARMD10; CD284; lipopolysaccharide response; Lps; Rasl2-8; TLR-4; TOLL
Chromosome: 4
Strain of origin: C57BL/10ScN
Molecular note: This allele has been characterized by lack of mRNA owing to a deletion of the locus. 74723bp of genomic DNA sequence have been removed, encompassing only the Tlr4 gene.
General note: C57BL/10ScN, C57BL/10ScNJ, and C57BL10/ScCr mice carry this allele.