Mice that are homozygous null for Il15ra are lymphopenic as result of decreased proliferation and homing of lymphocytes to peripheral lymph nodes. Deficiencies are seen in levels of natural killer and T cells.
Averil Ma, University of California, San Francisco
Genetic Background | Generation |
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N?+N2F34
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Il15ra | interleukin 15 receptor, alpha chain |
Il15ra mediates the high-affinity binding of Il15, a pleiotropic cytokine implicated in the development of innate immune cells. Mice that are homozygous null for Il15ra are viable and fertile. They are lymphopenic as result of decreased proliferation and homing of lymphocytes to peripheral lymph nodes. As a result, marked hypocellularity of lymph nodes is observed. Deficiencies are seen in levels of natural killer cells, natural killer T cells, CD8+ T lymphocytes, TCR delta/gamma intraepithelial lymphocytes and memory phenotype CD8+ T cells.
Loss of IL15RA expression has also been shown to induce a functional oxidative shift in fast muscles, substantially increasing fatigue resistance and exercises capacity. Knockout mice run greater distances and have greater ambulatory activity than control animals.
A targeting construct containing a phosphoglyceraldehyde driven neomycin resistance cassette flanked by loxP sequences and a phosphoglyceraldehyde driven thymidine kinase gene cassette was used to replace exons 2 and 3 of the Il15ra gene. The construct was electroporated into RW-4 ES ES cells. Selected ES cells were injected into C57BL/6 blastocysts.
Allele Name | targeted mutation 1, Averil Ma |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Il-15R-; Il15ra-; IL-15Ralpha-; IL-15Ralpha-; RalphaKO |
Gene Symbol and Name | Il15ra, interleukin 15 receptor, alpha chain |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 2 |
Molecular Note | A genomic fragment containing exons 2 and 3 was replaced by a neomycin resistance cassette. These sequences encode the ligand binding and proline/threonine rich extracellular domains. Northern blot analysis demonstrated that the transcript was not detectable in liver RNA from homozygous mice. |
Mutations Made By | Averil Ma, University of California, San Francisco |
This mutant originated on a B6;129 background. The donating investigator maintained it as a heterozygote using intercrosses. Coat color expected from breeding:Albino
When using the B6;129X1-Il15ratm1Ama/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #003723 in your Materials and Methods section.
Service/Product | Description | Price |
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Heterozygous for Il15ra<tm1Ama> |
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