Overview

MSM/Ms was derived from wild mice trapped in Mishima, Shizuoka Prefecture, Japan. MSM exhibits resistance to the lymphoma development and carries a Tlr3 variant that results in hypo-responsiveness to poly(I:C) stimulation. Wild-derived mice are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics and are valuable tools for genetic mapping, evolution and systematics research.

Genetic overview

Genetic Background	Generation
	F67+48 (2021-01-11 00:00:00)

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Research Applications

Immunology, Inflammation and Autoimmunity Research
Research Tools

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Base Price

Starting at:

$247.50 Domestic price for female 4-week

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Details

Detailed Description

MSM/Ms was derived from wild mice trapped in Mishima in Shizuoka Prefecture, Japan. This strain has been reported to be resistant to the development of lymphoma, due to at least two loci in crosses involving strain SL/Kh (Pataer et. al., 1996). C3HxMSM F1 hybrids treated with N-methyl-N-nitrosourea (MNU) develop squamous cell carcinomas of the forestomach with about 20% and 15% having mutations in H-ras and p53, respectively (Masui et. al., 1997). Stephan et. al. reported that wild-derived strains, MSM, CZECH (Stock No. 001144) and MOLF (Stock No. 000550), exhibit decreased secretion of TNF in response to stimulation by poly(I:C) (a synthetic analog of double-stranded RNA), the result of a P369L substitution in Tlr3. Response to Tlr2, Tlr4 and Tlr7-agonists is normal. Wild-derived mice are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics and are valuable tools for genetic mapping, evolution and systematics research.

In 2019-2020, researchers at The Jackson Laboratory discovered this inbred strain contains the Trem2^S148E allele - a naturally occurring variant at position 48351151-48351152 on Chr 17 (rs108080490 and rs107649577; Ensembl GRCm38.p6). This TC to GA transition results in a serine to glutamic acid substitution at amino acid 148 (S148E).

Development

The MSM inbred strain was derived from 6 female and 6 male Mus musculus molossinus mice trapped in the house of a physician, Dr. Segawa, in Mishima city, Shizuoka Prefecture, Japan in April and December, 1978, and maintained by Dr Kazuo Moriwaki (Moriwaki et al., 2009). This was closed colony bred for a year and in 1979 sibling inbreeding began. In 1986 this reached F20, an incipient inbred strain, and was designated MSM/Ms. Mice at inbreeding generation F67 were imported into The Jackson Laboratory in May, 2000, from Dr Moriwaki's colleague, Professor Toshihiko Shiroishi. In 2021 this strain reached generation F67+48.

Selected References

Masui T; Tezuka N; Nakanishi H; Inada K; Miyashita N; Tatematsu M. 1997. Induction of invasive squamous cell carcinomas in the forestomach of (C3H x MSM)F1, MSM, and C3H mice by N-methyl-N-nitrosourea and mutational analysis of the H-ras and p53 genes. Cancer Lett 111(1-2):97-104PubMed: 9022133 MGI: J:38729
Moriwaki K; Miyashita N; Mita A; Gotoh H; Tsuchiya K; Kato H; Mekada K; Noro C; Oota S; Yoshiki A; Obata Y; Yonekawa H; Shiroishi T. 2009. Unique inbred strain MSM/Ms established from the Japanese wild mouse. Exp Anim 58(2):123-34PubMed: 19448335 MGI: J:261873
Okumoto M; Mori N; Miyashita N; Moriwaki K; Imai S; Haga S; Hiroishi S; Takamori Y; Esaki K. 1995. Radiation-induced lymphomas in MSM, (BALB/cHeA x MSM) F1 and (BALB/cHeA x STS/A) F1 hybrid mice. Jikken Dobutsu 44(1):43-8PubMed: 7705478 MGI: J:22782
Pataer A; Kamoto T; Lu LM; Yamada Y; Hiai H. 1996. Two dominant host resistance genes to pre-B lymphoma in wild-derived inbred mouse strain MSM/Ms. Cancer Res 56(16):3716-20PubMed: 8706013 MGI: J:34941
Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11PubMed: 15081119 MGI: J:89298
Stephan K; Smirnova I; Jacque B; Poltorak A. 2007. Genetic analysis of the innate immune responses in wild-derived inbred strains of mice. Eur J Immunol 37(1):212-23PubMed: 17163447 MGI: J:117078
Yuasa T; Abe E; Oshimura M; Namba M. 1996. Immortalization of cells from brains derived from a strain (MSM/MSfB6C3F1) of wild mouse. Acta Med Okayama 50(6):319-24PubMed: 8985469 MGI: J:109906

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Genetics

Hbb^p

Allele Symbol: Hbb^p

Allele Name	p
Allele Type	Not Applicable
Allele Synonym(s)
Gene Symbol and Name	Hbb, hemoglobin beta chain complex
Gene Synonym(s)
Strain of Origin	AU
Chromosome	7

Gpi1^a

Allele Symbol: Gpi1^a

Allele Name	a variant
Allele Type	Not Applicable
Allele Synonym(s)
Gene Symbol and Name	Gpi1, glucose phosphate isomerase 1
Gene Synonym(s)
Strain of Origin	Not Applicable
Chromosome	7
Molecular Note	This allele determines a slower migrating variant of the encoded enzyme and occurs in the wild and in strains 101/H, A/HeJ, AKR/J, BALB/cJ, C57BR/cdJ, C58/J, CE/J, DBA/2J, DE/J, DW/J, LP/J, MA/J, NZB, PL/J, P/J, RARF, RF/J, SJL/J, ST/bJ, and 129/J. Sequencing analysis identified four nucleotide differences between this allele and Gpi1^b. Two differences are silent, while a third difference at position nucleotide position 282 predicts an aspartate for the a allele and an asparagine for the b allele at amino acid position 94. A fourth difference in the 3' untranslated region was also noted.

Thy1^a

Allele Symbol: Thy1^a

Allele Name	a variant
Allele Type	Not Applicable
Allele Synonym(s)	thetaAKR; theta-AKR; Thy1.1; Thy-1.1; Thy1a
Gene Symbol and Name	Thy1, thymus cell antigen 1, theta
Gene Synonym(s)
Site of Expression	The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells.
Strain of Origin	Not Applicable
Chromosome	9
General Note	The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1^a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1^b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experiments designed to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that is the same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900).
Molecular Note	The allele Thy1^a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Gpi1^a related

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
- Genetics Research
  - Tissue/Cell Markers

Genotype: Thy1^a related

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - T cell specific surface marker
- Genetics Research
  - Tissue/Cell Markers: T cell specific surface marker
  - Tissue/Cell Markers

Research Tools
- Genetics Research
  - Evolution and Systematics Research
  - Gene Mapping: Polymorphisms
  - Gene Mapping
- Cancer Research
  - genes regulating lymphoma development

Mammalian Phenotype Terms by Genotype

Phenotype Information

References

Masui T; Tezuka N; Nakanishi H; Inada K; Miyashita N; Tatematsu M. 1997. Induction of invasive squamous cell carcinomas in the forestomach of (C3H x MSM)F1, MSM, and C3H mice by N-methyl-N-nitrosourea and mutational analysis of the H-ras and p53 genes. Cancer Lett 111(1-2):97-104PubMed: 9022133 MGI: J:38729
Moriwaki K; Miyashita N; Mita A; Gotoh H; Tsuchiya K; Kato H; Mekada K; Noro C; Oota S; Yoshiki A; Obata Y; Yonekawa H; Shiroishi T. 2009. Unique inbred strain MSM/Ms established from the Japanese wild mouse. Exp Anim 58(2):123-34PubMed: 19448335 MGI: J:261873
Okumoto M; Mori N; Miyashita N; Moriwaki K; Imai S; Haga S; Hiroishi S; Takamori Y; Esaki K. 1995. Radiation-induced lymphomas in MSM, (BALB/cHeA x MSM) F1 and (BALB/cHeA x STS/A) F1 hybrid mice. Jikken Dobutsu 44(1):43-8PubMed: 7705478 MGI: J:22782
Pataer A; Kamoto T; Lu LM; Yamada Y; Hiai H. 1996. Two dominant host resistance genes to pre-B lymphoma in wild-derived inbred mouse strain MSM/Ms. Cancer Res 56(16):3716-20PubMed: 8706013 MGI: J:34941
Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11PubMed: 15081119 MGI: J:89298
Stephan K; Smirnova I; Jacque B; Poltorak A. 2007. Genetic analysis of the innate immune responses in wild-derived inbred strains of mice. Eur J Immunol 37(1):212-23PubMed: 17163447 MGI: J:117078
Yuasa T; Abe E; Oshimura M; Namba M. 1996. Immortalization of cells from brains derived from a strain (MSM/MSfB6C3F1) of wild mouse. Acta Med Okayama 50(6):319-24PubMed: 8985469 MGI: J:109906

Additional References

Kikkawa Y; Miura I; Takahama S; Wakana S; Yamazaki Y; Moriwaki K; Shiroishi T; Yonekawa H. 2001. Microsatellite database for MSM/Ms and JF1/Ms, molossinus-derived inbred strains. Mamm Genome 12(9):750-2PubMed: 11641725 MGI: J:71416
Sugimoto M; Abe K. 2007. X chromosome reactivation initiates in nascent primordial germ cells in mice. PLoS Genet 3(7):e116PubMed: 17676999 MGI: J:123745

Additional - Gpi1^a related

Additional - Hbb^p related

Additional - Thy1^a related

Technical Support

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Genotyping Protocols
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Dietary Information
- LabDiet® 5K54 formulation (4% fat)
Mating System
- Sibling x Sibling
Appearance
- white-bellied agouti
  Related Genotype: A^w/A^w
Citation
When using the MSM/MsJ mouse strain in a publication, please include JAX stock #003719 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX9 (Standard)

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Genetic overview

Research Applications

Base Price

Detailed Description

Development

Selected References

Hbbp

Allele Symbol: Hbbp

Gpi1a

Allele Symbol: Gpi1a

Thy1a

Allele Symbol: Thy1a