MSM/MsJ

Stock number: 003719
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

MSM/Ms was derived from wild mice trapped in Mishima, Shizuoka Prefecture, Japan. MSM exhibits resistance to the lymphoma development and carries a Tlr3 variant that results in hypo-responsiveness to poly(I:C) stimulation. Wild-derived mice are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics and are valuable tools for genetic mapping, evolution and systematics research.

Detailed description

MSM/Ms was derived from wild mice trapped in Mishima in Shizuoka Prefecture, Japan. This strain has been reported to be resistant to the development of lymphoma, due to at least two loci in crosses involving strain SL/Kh (Pataer et. al., 1996). C3HxMSM F1 hybrids treated with N-methyl-N-nitrosourea (MNU) develop squamous cell carcinomas of the forestomach with about 20% and 15% having mutations in H-ras and p53, respectively (Masui et. al., 1997). Stephan et. al. reported that wild-derived strains, MSM, CZECH (Stock No. 001144) and MOLF (Stock No. 000550), exhibit decreased secretion of TNF in response to stimulation by poly(I:C) (a synthetic analog of double-stranded RNA), the result of a P369L substitution in Tlr3. Response to Tlr2, Tlr4 and Tlr7-agonists is normal. Wild-derived mice are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics and are valuable tools for genetic mapping, evolution and systematics research.

In 2019-2020, researchers at The Jackson Laboratory discovered this inbred strain contains the Trem2^S148E allele - a naturally occurring variant at position 48351151-48351152 on Chr 17 (rs108080490 and rs107649577; Ensembl GRCm38.p6). This TC to GA transition results in a serine to glutamic acid substitution at amino acid 148 (S148E).

Development

The MSM inbred strain was derived from 6 female and 6 male Mus musculus molossinus mice trapped in the house of a physician, Dr. Segawa, in Mishima city, Shizuoka Prefecture, Japan in April and December, 1978, and maintained by Dr Kazuo Moriwaki (Moriwaki et al., 2009). This was closed colony bred for a year and in 1979 sibling inbreeding began. In 1986 this reached F20, an incipient inbred strain, and was designated MSM/Ms. Mice at inbreeding generation F67 were imported into The Jackson Laboratory in May, 2000, from Dr Moriwaki's colleague, Professor Toshihiko Shiroishi. In 2021 this strain reached generation F67+48.

Allele

Symbol: Hbb^p
Name: p
MGI accession ID: MGI:1858120
Generation method: Not Applicable
Marker symbol: Hbb
Marker name: hemoglobin beta chain complex
Chromosome: 7
Strain of origin: AU

Allele

Symbol: Thy1^a
Name: a variant
MGI accession ID: MGI:3579311
Generation method: Not Applicable
Marker symbol: Thy1
Marker name: thymus cell antigen 1, theta
Chromosome: 9
Strain of origin: Not Applicable
Site of expression: The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells.
Molecular note: The allele Thy1^a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains.
General note:

The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1^a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1^b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experiments designed to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that is the same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900).

Allele

Symbol: Gpi1^a
Name: a variant
MGI accession ID: MGI:2177271
Generation method: Not Applicable
Marker symbol: Gpi1
Marker name: glucose phosphate isomerase 1
Chromosome: 7
Strain of origin: Not Applicable
Molecular note: This allele determines a slower migrating variant of the encoded enzyme and occurs in the wild and in strains 101/H, A/HeJ, AKR/J, BALB/cJ, C57BR/cdJ, C58/J, CE/J, DBA/2J, DE/J, DW/J, LP/J, MA/J, NZB, PL/J, P/J, RARF, RF/J, SJL/J, ST/bJ, and 129/J. Sequencing analysis identified four nucleotide differences between this allele and Gpi1^b. Two differences are silent, while a third difference at position nucleotide position 282 predicts an aspartate for the a allele and an asparagine for the b allele at amino acid position 94. A fourth difference in the 3' untranslated region was also noted.