B6.Cg-Tg(Thy1-CFP)23Jrs/J

Stock number: 003710
Research areas: Neurobiology Research, Research Tools

Description

These transgenic mice express fluorescence in motor and sensory neurons and subsets of central neurons. This strain may be useful for double-labeling applications, or breeding to other YFP/GFP lines.

Detailed description

These mice express a spectral variant of GFP (cyan-CFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. This line provides a strong and specific vital marker for axons; fluorescence is also stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood. Availability of multiple spectral variants is useful for double-labeling applications, or breeding to other YFP/GFP lines.

Development

A transgenic construct containing a CFP gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized B6CBAF1 mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder animals were obtained and crossed with wildtype C57BL/6J mice.

Allele

Symbol: Tg(Thy1-CFP)23Jrs
Name: transgene insertion 23, Joshua R Sanes
MGI accession ID: MGI:3505582
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Thy1-CFP)23Jrs
Marker name: transgene insertion 23, Joshua R Sanes
Chromosome: UN
Strain of origin: (C57BL/6J x CBA)F1
Expressed genes: CFP,Cyan Fluorescent Protein,jellyfish
Site of expression: High levels of expression in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in non-neural cells.
Molecular note: The transgene contains a CFP gene under the direction of regulatory elements derived from the mouse Thy1 gene. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. Lines 4, 11, 23, D, F, H, I, and S were established.
General note: Transgenic mice express a spectral variant of GFP (cyan-CFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. Fluorescence is stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood.