These transgenic mice express fluorescence in motor and sensory neurons and subsets of central neurons. This strain may be useful for double-labeling applications, or breeding to other YFP/GFP lines.
Joshua R Sanes, Harvard University
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type |
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Transgenic (Reporter) |
These mice express a spectral variant of GFP (cyan-CFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. This line provides a strong and specific vital marker for axons; fluorescence is also stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood. Availability of multiple spectral variants is useful for double-labeling applications, or breeding to other YFP/GFP lines.
A transgenic construct containing a CFP gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized B6CBAF1 mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder animals were obtained and crossed with wildtype C57BL/6J mice.
Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish |
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Site of Expression | High levels of expression in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in non-neural cells. |
Allele Name | transgene insertion 23, Joshua R Sanes |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Thy-1-CFP |
Gene Symbol and Name | Tg(Thy1-CFP)23Jrs, transgene insertion 23, Joshua R Sanes |
Gene Synonym(s) | |
Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish |
Site of Expression | High levels of expression in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in non-neural cells. |
Strain of Origin | (C57BL/6J x CBA)F1 |
Chromosome | UN |
General Note | Transgenic mice express a spectral variant of GFP (cyan-CFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. Fluorescence is stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood. |
Molecular Note | The transgene contains a CFP gene under the direction of regulatory elements derived from the mouse Thy1 gene. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. Lines 4, 11, 23, D, F, H, I, and S were established. |
Mutations Made By | Joshua Sanes, Harvard University |
This strain originated on a mixed B6CBAF1 background. The donating investigator has been backcrossing the strain to C57BL/6 and indicated that it was at N6 (4/14/00). Coat color expected from breeding:Black
When using the B6.Cg-Tg(Thy1-CFP)23Jrs/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #003710 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Thy1-CFP)23Jrs |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-CFP)23Jrs/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-CFP)23Jrs/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-CFP)23Jrs/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-CFP)23Jrs/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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