| Allele Name | transgene insertion 23, Joshua R Sanes |
| Allele Type | Transgenic (Reporter) |
| Allele Synonym(s) | Thy-1-CFP |
| Gene Symbol and Name | Tg(Thy1-CFP)23Jrs, transgene insertion 23, Joshua R Sanes |
| Gene Synonym(s) | |
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
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| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish |
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| Site of Expression | High levels of expression in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in non-neural cells. |
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| Strain of Origin | (C57BL/6J x CBA)F1 |
| Chromosome | UN |
| General Note | Transgenic mice express a spectral variant of GFP (cyan-CFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. Fluorescence is stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood. |
| Molecular Note | The transgene contains a CFP gene under the direction of regulatory elements derived from the mouse Thy1 gene. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. Lines 4, 11, 23, D, F, H, I, and S were established. |
| Mutations Made By | Joshua Sanes, Harvard University |
