These PB-CDC37.1 mice express a transgene containing Cdc37 under the direction of a probasin promoter with males exhibiting prostatic growth-related disturbances.
Dr. Lilia Stepanova, St. Jude Children's Research Hospital
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
This strain expresses Cdc37 under the direction of a probasin promoter resulting in over expression in prostatic epithelia, a tissue where the Cdc37 gene product is absent or present at low levels. While less than ten percent of wildtype littermates develop some degree of hyperplasia by twelve months of age, eighty percent of these transgenic mice exhibit prostatic growth-related disturbances by eight months. Expression is limited to the ventral lobe of the prostate. Correspondingly, hyperplasia is restricted to the ventral prostate. Hyperplasia is frequently accompanied by necrosis. The transgenic insertion site is assumed to be on the Y chromosome as all transgenics are male.
A plasmid containing a Pbsn promoter, Cdc37 open reading frame and SV40 Tag splice site/polyadenylation signal sequence was injected into male pronuclei of B6D2F1 mouse embryos. Founder animals were mated with ICR mice.
Expressed Gene | Cdc37, cell division cycle 37, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 1, Lilia Stepanova |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | PB-CDC37.1 |
Gene Symbol and Name | Tg(Pbsn-Cdc37)1Stp, transgene insertion 1, Lilia Stepanova |
Gene Synonym(s) | |
Promoter | Pbsn, probasin, rat |
Expressed Gene | Cdc37, cell division cycle 37, mouse, laboratory |
Strain of Origin | (C57BL/6 x DBA/2)F1 |
Chromosome | UN |
Molecular Note | The transgene contains a rat probasin promoter, a mouse Cdc37 open reading frame, and SV40 Tag splice site/polyadenylation signal sequence. The transgenic insertion site is assumed to be on the Y chromosome as all transgenic mice are male. Transgenic mice overexpress Cdc37 in prostatic epithelia, a tissue where the endogenous Cdc37 gene product is absent or present at low levels. |
Mutations Made By | Dr. Lilia Stepanova, St. Jude Children's Research Hospital |
This strain originated on a B6D2F1 background and was crossed to an ICR strain. It is being described as STOCK. The transgenic insertion site is assumed to be on the Y chromosome as all transgenics are male.It is maintained as a hemizygote. Hemizygous males and +/+ females will be offered for sales.
When using the PB-CDC37.1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #003689 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Pbsn-Cdc37)1Stp |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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