Jackson Laboratory SEARCH FOR MICE
B6.C3Ga-Mfrprd6/J
Stock No: 003684
  • Congenic
Contact Customer Service
Contact Customer Service
  • Email
  • Download PDF
  • Print
  • Help
  • Overview
  • Details
    • Detailed Description
    • Development
    • Control Suggestions
  • Genetics
  • Disease/Phenotype
    • Disease Terms
    • Research Areas By Phenotype
    • Mammalian Phenotype Terms by Genotype
    • References
  • Technical Support
    • Genotyping Protocols
    • Mating System
    • Breeder Pairs
    • Appearance
    • Citation
    • Animal Health Reports
    • Contact Technical Support
  • Pricing & Availability
    • Price
    • Payment Terms
    • Related Products and Services
  • Terms Of Use
  • Related Strains

Overview

Male and female mice homozygous for rd6develop a slowly progressive retinal degeneration affecting both rod and cone cells beginning at 3-4 weeks of age, soon after the retina develops. This mutant has been suggested as a model for the human flecked retinal disorder retinitis punctata albicans (RPA), which causes progressive visual loss similar to that characteristic of retinitis pigmentosa.

Read More +

Genetic overview

Genetic Background Generation
000664 C57BL/6J
NE10F?+41
(2020-04-23 00:00:00)

Mfrprd6

Allele Type Gene Symbol Gene Name
Spontaneous Mfrp membrane frizzled-related protein
View Genetics

Research Applications

  • Sensorineural Research
  • Research Tools
View All Research Applications

Base Price

Starting at:

$136.70 Domestic price for female
View Price List

Details

Detailed Description

Male and female mice homozygous for rd6develop a slowly progressive retinal degeneration affecting both rod and cone cells beginning at 3-4 weeks of age, soon after the retina develops. Ophthalmoscopic examination reveals small, evenly distributed white spots throughout the retina from the time the mice are 8 weeks old. The spots remain clearly visible until about 7 months, when clinical signs of retinal degeneration first become evident, and become less easily distinguishable as degeneration progresses. Retinal blood vessels appear pale and attenuated by 7 months and are undetectable by 15 months, when the fundus appears mottled, lightly pigmented and granular. Aberrations of the photoreceptor layer are histologically evident at 3-4 weeks; by one year, only one to three cell layers (of the normal 10-12) remain. The time and distribution of the ophthalmoscopically detectable retinal spots correlate with histologic observation of putative phagocytic cells in the subretinal space. This mutant has been suggested as a model for the human flecked retinal disorder retinitis punctata albicans (RPA), which causes progressive visual loss similar to that characteristic of retinitis pigmentosa. (Hawes et al. 2000)

Development

The Catb (catalase 1 null) mutation was identified in a screen for low catalase activity by Feinstein et al. (1964, 1966) at Argonne National Laboratory of offpring of (C3H x 101) male mice irradiated at Oak Ridge National Laboratory (600 R in fractionated exposures), then crossed to females of the Oak Ridge "multiple recessive testing stock" derived from a cross of NB mice (aabbcch p/cch p d se/d se) and mice of a non-inbred stock homozygous for three of the same recessive mutations, as well as for s, from which mice homozygous for all seven mutations were bred and maintained afterward by random breeding (Russell 1951). Putative heterozygotes for catalase deficiency were crossed to normal mice (not clearly identified, but presumably unirradiated progeny of (101 x C3H)F1 X ORNL testing stock), and offspring exhibiting low catalase activity were backcrossed to the heterozygous parent (Feinstein et al. 1966).Catb was subsequently transferred by backcrossing for eight generations onto C3H/HeAnl, a line carrying mouse mammary tumor virus (MMTV) and another line (C3Hf) free of the virus (Feinstein et al. 1978). The Jackson Laboratory received C3HfB/Ga-Catb, now C3Ga.Cg-Catb/J (stock #001906), from Allen H. Gates, then at the University of Rochester, in 1982.

Although all C3H strains carry the rd1 (retinal degeneration 1) mutation at Pde6b, the gene encoding the beta subunit of phosphodiesterase 1, retinas of these mice exhibited a peculiar, granular appearance distinct from the usual Pde6brd1 phenotype. F2 progeny of a cross of C3HfB/Ga-Catb and C57BL/6J mice exhibited three retinal phenotypes: 1) normal; 2) patches of pigment deposition and large, diffuse pigmented regions typical Pde6brd1 homozygotes; and 3) small, discrete, light-colored dots throughout the fundus. Mice exhibiting the last phenotype were intercrossed among themselves to produce a stock homozygous for Mfrprd6 and for the wild-type allele at Pde6b. During this process, a separate mutation with a distinct map position was identified and characterized as Crb1rd8. STOCK Crb1rd8 Mfrprd6/J (stock #004299) is homozygous for both Mfrprd6 and Crb1rd8 on this mixed genetic background that derived in part from C57BL/6J, C3H, 101, and a linkage testing stock derived from non-inbred stocks. In order to isolate Mfrprd6 and Crb1rd8 this strain was backcrossed to C57BL/6J, and selection for either Mfrprd6 or Crb1rd8 yielded B6.C3Ga-Mfrprd6/J (stock #003684) and STOCK Crb1rd8/J (stock #003392) respectively.

Control Suggestions

  • 000664 C57BL/6J

Additional Information

  • Considerations for Choosing Controls

Genetics

Mfrprd6

Allele Symbol: Mfrprd6

Allele Name retinal degeneration 6
Allele Type Spontaneous
Allele Synonym(s)
Gene Symbol and Name Mfrp, membrane frizzled-related protein
Gene Synonym(s)
Strain of Origin C3fBAnl.Cg-Catb/AnlJ
Chromosome 9
Molecular Note The rd6 mutation was identified as a 4 bp deletion in the splice donor sequence of intron 4 in the Mfrp gene. The mutation results in the skipping of exon 4. While the mutation does not cause a frameshift, 58 amino acids are deleted from the protein.

Disease/Phenotype

Disease Terms

Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).

  • fundus albipunctatus

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Mfrprd6 related

  • Sensorineural Research
    • Retinal Degeneration
      • retinal spots
  • Research Tools
    • Sensorineural Research
      • retinal spots

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain

Genotype: Mfrprd6/Mfrprd6
involves: C3HfB6/Anl

mammalian phenotype

  • vision/eye phenotype
    • Normal - retinal pigment epithelium phagocytosis is normal
    • (MGI Ref ID J:181596)

pigmentation phenotype

  • abnormal retinal pigment epithelium morphology
    • mice exhibit an increase in the number of apical microvilli compared with wild-type mice
    • (MGI Ref ID J:181596)

vision/eye phenotype

  • abnormal retinal pigment epithelium morphology
    • mice exhibit an increase in the number of apical microvilli compared with wild-type mice
    • (MGI Ref ID J:181596)
The following phenotype relates to a compound genotype created using this strain

Genotype: Mfrprd6/Mfrprd6
B6.C3-Mfrp

nervous system phenotype

  • retinal photoreceptor degeneration
    • progressive degeneration of rods and cones as detected by electroretinogram; age of onset is approximately 1 month
    • (MGI Ref ID J:64087)

vision/eye phenotype

  • retinal spots
    • subretinal spots oriented in a regular pattern across the retina are apparent by ophthalmoscopic examination at approximately 8-10 weeks of age
    • (MGI Ref ID J:64087)
  • retinal degeneration
    • age of onset is approximately 7 months
    • (MGI Ref ID J:64087)
  • retinal photoreceptor degeneration
    • progressive degeneration of rods and cones as detected by electroretinogram; age of onset is approximately 1 month
    • (MGI Ref ID J:64087)

References

Additional References

  • Chang B; Hawes NL; Hurd RE; Davisson MT; Nusinowitz S; Heckenlively JR. 2002. Retinal degeneration mutants in the mouse. Vision Res 42(4):517-25PubMed: 11853768MGI: J:75095
  • Feinstein RN; Fry RJ; Staffeldt EF. 1978. Carcinogenic and antitumor effects of aminotriazole on acatalasemic and normal catalase mice. J Natl Cancer Inst 60(5):1113-6PubMed: 642030MGI: J:5971
  • Feinstein RN; Howard JB; Braun JT; Seaholm JE. 1966. Acatalasemic and hypocatalasemic mouse mutants. Genetics 53(5):923-33PubMed: 5929246MGI: J:5015
  • Hawes NL; Chang B; Hageman GS; Nusinowitz S; Nishina PM; Schneider BS; Smith RS; Roderick TH; Davisson MT; Heckenlively JR. 2000. Retinal degeneration 6 (rd6): a new mouse model for human retinitis punctata albescens. Invest Ophthalmol Vis Sci 41(10):3149-57PubMed: 10967077MGI: J:64087
  • Kameya S; Hawes NL; Chang B; Heckenlively JR; Naggert JK; Nishina PM. 2002. Mfrp, a gene encoding a frizzled related protein, is mutated in the mouse retinal degeneration 6. Hum Mol Genet 11(16):1879-86PubMed: 12140190MGI: J:78123

Additional - Mfrprd6 related

Technical Support

CONTACT TECHNICAL SUPPORT
  • Genotyping Protocols

    • Sanger sequencing:Mfrp
    • Probe:Mfrp Probe
    • Genotyping resources and troubleshooting
  • Mating System

    • Homozygote x Homozygote
  • Appearance

    • black, retinal spots
      Related Genotype: a/a, Mfrprd6/Mfrprd6
  • Citation

    When using the B6.C3Ga-Mfrprd6/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #003684 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

MGL277 (Low)

Pricing & Availability

Availability Varies
  • Domestic
  • International

Live Mouse

Age Genotype Price
weeks

Cryorecovery - Pricing

Service/Product Description Price
Homozygous for Mfrp<rd6>, 1 pair minimum

Related Products and Services

Frozen Mouse EmbryoB6.C3Ga-Mfrp<rd6>/J Frozen Embryos $2595.00
Frozen Mouse EmbryoB6.C3Ga-Mfrp<rd6>/J Frozen Embryos $2595.00
Frozen Mouse EmbryoB6.C3Ga-Mfrp<rd6>/J Frozen Embryos $3373.50
Frozen Mouse EmbryoB6.C3Ga-Mfrp<rd6>/J Frozen Embryos $3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470
Email: TechTran@jax.org

Related Strains

  • All
  • By Allele
  • By Gene
  • By Collection
View All Strains
There are no related strains using this filter.
▲
  • Stock No: |
    Related By:
▼
FEEDBACK
Did you find what you were looking for?

What information were you hoping to find through your search?

How easy was it to find what you were looking for?

We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.

Please Enter a Valid Email Address

Skip

Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.