These Gad2 knock-out mice exhibit stress-generated seizures. They are suitable for use in applications related to the study of epilepsy.
Steinunn Baekkeskov, UCSF
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Gad2 | glutamic acid decarboxylase 2 |
Gad2-null mice are viable and fertile. Gross appearance is similar to wildtype littermates; brain weight is unaffected, as is cytoarchitecture. Starting as early as 12 weeks of age, homozygous-null mice develop stress-generated seizures induced by cage lid removal and other routine handling. This epileptic syndrome results in increased mortality with 70% of mice failing to survive to 7 months of age.
Other phenotypic observations
* defect in the plastcicity of the visual cortex during development
* increased anxiety-like responses in open field and elevated zero maze assays
* diminished response to anxiolytics (anti-anxiety agents)
* Defect in the ability to sustain maximum gamma-aminobutyric acid release during stimulation
Genetic background influences this strain's phenotype. Homozygous-null mice on a mixed NOD,129 background exhibit a higher incidence of apparently less severe seizures than mice on a B6,129 background (also see Stock No. 003653).
Exon 1 is disrupted in this strain. A targeting vector containing the neomycin resistance gene under the control of the phosphoglycerol kinase promoter was introduced into the BamHI site in exon 1 just downstream of the transcription and translation start sites. The construct was electroporated into 129X1/SvJ-derived JM1 embryonic stem cells and selected cells were injected into C57BL/6 blastocysts. No protein product was detected in brain extracts of homozygous-null mice using Western blots.
Allele Name | targeted mutation 1, Steinunn Baekkeskov |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | GAD 65 (-); GAD65 - |
Gene Symbol and Name | Gad2, glutamic acid decarboxylase 2 |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 2 |
General Note | Phenotypic Similarity to Human Syndrome: Epilepsy (J:44638). |
Molecular Note | A neomycin resistance cassette was inserted into exon 1, downstream of the translation initiation start site. Western blot analysis on brain extracts from homozygous mice confirmed that no detectable protein is expressed from this allele. |
Mutations Made By | Steinunn Baekkeskov, UCSF |
The expected coat color is black.
When using the B6-GAD65- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003654 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Gad2<tm1Bae> |
Frozen Mouse Embryo | B6.129X1-Gad2<tm1Bae>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Gad2<tm1Bae>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Gad2<tm1Bae>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129X1-Gad2<tm1Bae>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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