A C57BL/6J-congenic version of this strain is available as Stock No. 029661.
These knock-out mice, susceptible to infection, are useful when studying the role of C3 during immune responses.
Michael C. Carroll, The Center for Blood Research
Genetic Background | Generation |
---|---|
N?+pN7F27
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | C3 | complement component 3 |
Mice homozygous for the C3 (complement component C3) targeted mutation are viable and fertile. Homozygous mutants exhibit an increased susceptibility to lethal infection by Group B streptococci. Reductions in peritoneal mast cell degranulation, production of tumor necrosis factor alpha, neutrophil infiltration and bacterial clearance have also been reported in these mice. Homozygotes also demonstrate a profound defect in antibody response to T cell dependent antigens. They show a diminished level of peanut agglutinin+ germinal centers and a failure in isotype switching despite normal B cell signaling in vitro.
The C3 gene is disrupted by PGK/Neo cassette. Approximately 600 nt of the gene are deleted. A portion of these nts (nt 1850-2214; AA 620-741, pro-C3 numbering) fall within the coding region. The targeting construct was transfected into 129S4/SvJae derived J1 ES cells. Successful transfectants were injected into 3.5 day old C57BL/6 blastocysts which were then implanted into the uterus of pseudopregnant females. Male chimeric mice were bred with C57BL/6 females. The donating investigator reported backcrossing this strain to C57BL/6J for at least five generations prior to sending to The Jackson Laboratory Repository (see SNP notes below).
A 32 SNP (single nucleotide polymorphism) panel analysis, with markers covering all 19 chromosomes and the X chromosome, was performed on the rederived living colony at The Jackson Laboratory Repository. This revealed 3 markers (representing one each on chromosomes 7, 12, and 19) that were not fixed for C57BL/6 allele-type (e.g.: still segregating for 129S4 allele-type markers). The marker on chromosome 12 may be fixed as homozygous for the 129S4 allele-type. These data suggest the mice may have been backcrossed to C57BL/6 for 1-2 fewer generations than reported prior to arrival at The Jackson Laboratory Repository.
Allele Name | targeted mutation 1, Michael C Carroll |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | C3tm1Crr; C3-; mC3- |
Gene Symbol and Name | C3, complement component 3 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 17 |
Molecular Note | Insertion of a PGK-neomycin resistance cassette into an exon of the C3 gene deleted sequences that code for the C-terminal region of the beta chain and the N-terminal region of the alpha chain, including the site for processing the pro-C3 molecule. ELISA testing did not detect C3 protein in serum of homozygous mutant mice. A C3 hemolytic assay did not detect functional C3 activity. |
Mutations Made By | Michael Carroll, The Center for Blood Research |
When maintaining a live colony, homozygous mice may be bred together. The expected coat color from breeding is black.
When using the B6;129S4-C3tm1Crr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #003641 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for C3<tm1Crr> |
Frozen Mouse Embryo | B6;129S4-C3<tm1Crr>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S4-C3<tm1Crr>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S4-C3<tm1Crr>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;129S4-C3<tm1Crr>/J Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.