These Grm4 knock-out mice exhibit impairment of the ability to learn complex motor tasks and of paired-pulse facilitation and post-tetanic potentiation.
IMR Colony, The Jackson Laboratory
Metabotropic glutamate receptors are widely distributed throughout the central nervous system. Included in this group is the glutamate receptor, metabotropic 4 (also called mGluR4). The targeted gene product is found at modest levels in several areas of the CNS, but it appears to be expressed at higher levels in the granule cells of the cerebellum. No protein product is detected in the brain by immunological assays. Mutant mice appear normal in size/weight and have normal motor coordination but appear to have an impaired ability to learn complex motor tasks. The donating investigators note that these mice are selectively resistant to chemically-induced absence seizures.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. If necessary, we will modify the strain description as published results become available.
|Allele Name||targeted mutation 1, David R Hampson|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Gprc1dtm1Hpn; mGluR4-|
|Gene Symbol and Name||Grm4, glutamate receptor, metabotropic 4|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A neomycin resistance cassette was inserted into an EcoRV site of an exon that encodes a region encompassing Gly 246-Arg 291 of the putative amino terminal extracellular domain of the protein. Immunoblots of cerebella from homozygous mutant mice showed no detectable protein.|
|Mutations Made By|| |
David Hampson, University of Toronto