These Cd86 knock-out mice exhibit failure to form germinal centers upon intravenous immunization without adjuvant.
Arlene H Sharpe, Harvard Medical School
Cd86 (B7-2) null mice are viable and fertile. Unimmunized mice have normal levels of serum immunoglobulin and normal numbers of B and T lymphocytes. Upon intravenous immunization without adjuvant, they fail to form germinal centers or undergo isotype switching and antigen specific IgG1 and IgG2a isotypes are found to be 5% that of wild type levels. When the immunization route is subcutaneous, IgG1 and IgG2a levels are the same as in wild type mice.
A targeting vector containing a neomycin gene under the control of the mouse phosphoglycerol kinase (PGK) promoter was used to disrupt the exon encoding the Ig-V-like domain in J1 embryonic stem (ES) cells. ES cells were injected into C57BL/6 blastocysts.
SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N (3 of 4 markers segregating) in the development of the strain. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J at least once to establish the colony.
|Allele Name||targeted mutation 1, Arlene H Sharpe|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||B7-2-; B7.2 KO|
|Gene Symbol and Name||Cd86, CD86 antigen|
|Gene Synonym(s)||B7-2; B7.2; B70; CD28 antigen ligand; CD28LG2; Cd28l2; Cd28l2; LAB72; Ly-58; Ly-58; Ly58; Ly58; MB7-2; lymphocyte antigen 58|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A neomycin selection cassette replaced an exon encoding the IgV-like domain of the protein and flanking genomic sequences. FACS analysis on splenocytes and LPS-stimulated B cells derived from homozygous mice confirmed that no functional protein was encoded by this allele.|
|Mutations Made By|| |
Arlene Sharpe, Harvard Medical School
This strain originated on a B6/129S4 background. It was backcrossed to B6 for ten generations and has been maintained with intercrosses since N10. It is maintained as a homozygote. Coat color expected from breeding:Black
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
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