These Cd86 knock-out mice exhibit failure to form germinal centers upon intravenous immunization without adjuvant.
Arlene H Sharpe, Harvard Medical School
Cd86 (B7-2) null mice are viable and fertile. Unimmunized mice have normal levels of serum immunoglobulin and normal numbers of B and T lymphocytes. Upon intravenous immunization without adjuvant, they fail to form germinal centers or undergo isotype switching and antigen specific IgG1 and IgG2a isotypes are found to be 5% that of wild type levels. When the immunization route is subcutaneous, IgG1 and IgG2a levels are the same as in wild type mice.
A targeting vector containing a neomycin gene under the control of the mouse phosphoglycerol kinase (PGK) promoter was used to disrupt the exon encoding the Ig-V-like domain in J1 embryonic stem (ES) cells. ES cells were injected into C57BL/6 blastocysts.
SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N (3 of 4 markers segregating) in the development of the strain. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J at least once to establish the colony.
|Allele Name||targeted mutation 1, Arlene H Sharpe|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||B7.2 KO; B7-2-|
|Gene Symbol and Name||Cd86, CD86 antigen|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A neomycin selection cassette replaced an exon encoding the IgV-like domain of the protein and flanking genomic sequences. FACS analysis on splenocytes and LPS-stimulated B cells derived from homozygous mice confirmed that no functional protein was encoded by this allele.|
|Mutations Made By|| |
Arlene Sharpe, Harvard Medical School
This strain originated on a B6/129S4 background. It was backcrossed to B6 for ten generations and has been maintained with intercrosses since N10. It is maintained as a homozygote. Coat color expected from breeding:Black
When using the B7-2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003609 in your Materials and Methods section.