The RIP-Cre transgene has a 668 bp fragment of the rat insulin II promoter, nuclear-localized Cre recombinase, and a 2.1 kbp fragment from the human growth hormone gene. These RIP-Cre transgenic mice are a Cre-lox tool useful for deletion of floxed sequences in pancreatic beta cells.
Mark A. Magnuson, Vanderbilt University School of Medicine
Genetic Background | Generation |
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N13F4
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Allele Type |
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Transgenic (Recombinase-expressing) |
This strain carries the "RIP-Cre" transgene construct (containing a 668 bp fragment of the rat insulin II promoter, Cre recombinase with a nuclear localization sequence, and a 2.1 kb fragment from the human growth hormone gene). Hemizygous mice carrying this transgene are phenotypically normal and overexpresss cre specifically in pancreatic beta cells. This transgene strain is used in combination with mice carrying floxed targeted mutations to create various pancreatic beta cell-specific gene knockouts using the "Cre-lox" system. Results from several different laboratories have shown that this transgenic strain is at least 85% efficient in achieving pancreatic beta cell-specific recombination. It should also be noted that the transgene in this line has been found to be expressed at a low level in the hypothalamus. In some cases this has resulted in a phenotype due to deletion of the floxed allele in this region of the brain. It has also been shown that these transgenic mice may spontaneously develop glucose intolerance and impaired insulin secretion developing at 6-8 weeks of age. It is recommended that users include naive "RIP-Cre" mice (i.e., those not bred to a floxed mutant) among the controls used in experiments.
A transgenic construct containing a 668 bp rat insulin II promoter, nuclear localization sequence-modified Cre recombinase and a 2.1 kbp fragment from the human growth hormone gene was injected into B6D2(F2) pronuclei. The allele was subsequently moved to a C57BL/6J background.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | pancreatic beta cells |
Allele Name | transgene insertion 25, Mark A Magnuson |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | [RIP]-Cre; Ins2cre; Ins-Cre; M line; RIP Cre; RIP2Cre; RIP2-cre; RIPCre; Rip-cre; TgN(ins2-Cre)25Mgn |
Gene Symbol and Name | Tg(Ins2-cre)25Mgn, transgene insertion 25, Mark A Magnuson |
Gene Synonym(s) | |
Promoter | Ins2, insulin 2, rat |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | pancreatic beta cells |
Strain of Origin | (C57BL/6 x DBA)F2 |
Chromosome | 7 |
Molecular Note | This transgene expresses Cre recombinase with a human growth hormone polyadenylation signal under the control of the rat insulin promoter (Ins2), which is active in pancreatic beta cells and in neurons of the hypothalamus. 9 copies of the transgene are estimated to be present. Transgene insertion occurred on Chr 7, causing a 17400 bp duplication. |
When maintaining a live colony, these mice may be bred as homozygotes. Expected coat color from breeding is Black.
When using the Rip-cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #003573 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Ins2-cre)25Mgn |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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