On an albino background like this, the X-linked transgene Tg(Tyr)3412ARpw permits visual identification of gender as early as embryonic day 10.5.
Read More +Genetic Background | Generation |
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001800 FVB/NJ |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
This strain is segregating for Tg(Tyr)3412ARpw and homozygous for agouti (A) and albino (Tyrc). Mice of this strain that are carrying Tg(Tyr3412)ARpw are pigmented while those not carrying Tg(Tyr3412)ARpw are albino. On an albino background like this, the X-linked transgene Tg(Tyr)3412ARpw permits visual identification of sex as early as embryonic day 10.5 when eye pigment is discernible. Because Tg(Tyr)3412ARpw inserted into the X Chromosome, breeding a carrier male with a noncarrier (wild-type) female results in embryos in which all XX individuals develop coat and eye pigment, due to the Tg(Tyr)3412ARpw inherited from their father, while all XY individuals have a non-pigmented coat and eyes, having inherited a wild-type X Chromosome from their mother.
FVB/NJ-Tg(Tyr)3412ARpw/EiJ was generated by microinjecting the Tg(Tyr)3412ARpw construct into FVB/N fertilized eggs where Tg(Tyr)3412ARpw integrated into the X Chromosome. Selecting a pigmented transgene carrier at each generation, this strain was maintained by continuously backcrossing Tg(Tyr)3412ARpw to FVB/NJ. Embryos were generated for cryopreservation by mating hemizygous males at generation N6 to FVB/NJ females. All recovered females should carry the transgene and have a mottled pigmented coat due to random X–inactivation while all recovered males will lack the transgene and have an albino coat.
Expressed Gene | Tyr, tyrosinase, mouse, laboratory |
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Site of Expression | Fluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females. |
Allele Name | transgene insertion 3412A, Richard P Woychick |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Tg3412A |
Gene Symbol and Name | Tg(Tyr)3412ARpw, transgene insertion 3412A, Richard P Woychick |
Gene Synonym(s) | |
Promoter | Tyr, tyrosinase, mouse, laboratory |
Expressed Gene | Tyr, tyrosinase, mouse, laboratory |
Site of Expression | Fluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females. |
Strain of Origin | FVB/NJ |
Chromosome | X |
albino male
Related Genotype: A/A Tyrc/Tyrc +/Y
mottled gray-brown female
Related Genotype: A/A Tyrc/Tyrc Tg(Tyr)3412ARpw/+
dark gray-brown male
Related Genotype: A/A Tyrc/Tyrc Tg(Tyr)3412ARpw/Y
View images of coat colors for this strain.
When using the FVB/NJ-Tg(Tyr)3412ARpw/EiJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #003562 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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X linked- Hemizygous females and Non carrier males for Tg(Tyr)3412ARpw, 1 pair minimum |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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