These MMTV-Cre/Line A mice express a transgene containing Cre recombinase with widespread expression, including the female germline, under control the MMTV LTR promoter.
Dr. Lothar Hennighausen, National Institutes of Health
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
This transgenic strain expresses P1 Cre recombinase under the control of the MMTV LTR promoter. The MMTV LTR promoter directs a widespread pattern of expression including the female germline. High levels of recombination have been detected in the virgin and lactating mammary gland, salivary gland, seminal vesicle, skin, and various cells of the immune system. Little background recombination was observed in the lung, kidney, liver and brain tissues. The donating investigator indicates that this strain may be suitable for use in applications where it is desirable to delete genes in the virgin and lactating mammary gland, and other secretory tissues. Furthermore, since this strain expresses cre in the female germline, it can also be utilized to generate a knockout allele from a targeted locus with loxP sites with an efficiency of 100%.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | widespread pattern of expression including the female germline; high levels of recombination have been detected in the virgin and lactating mammary gland, salivary gland, seminal vesicle, and various cells of the immune system; little background recombination was observed in the lung, kidney, liver and brain tissues |
Allele Name | transgene insertion 1, Lothar Hennighausen |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | A1 line; A-line; CreA; MMTV Cre line A; MMTV-Cre; MMTV-CrelineA; MMTV-CreA |
Gene Symbol and Name | Tg(MMTV-cre)1Mam, transgene insertion 1, Lothar Hennighausen |
Gene Synonym(s) | |
Promoter | MMTV, Mouse Mammary Tumor Virus, MMTV |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | widespread pattern of expression including the female germline; high levels of recombination have been detected in the virgin and lactating mammary gland, salivary gland, seminal vesicle, and various cells of the immune system; little background recombination was observed in the lung, kidney, liver and brain tissues |
Strain of Origin | FVB |
Chromosome | UN |
General Note | This transgene is the A line. Line 4 (D) and line F were also produced. |
Molecular Note | This transgene directs the expression of Cre recombinase under the control of the mouse mammary tumor virus (MMTV) long terminal repeat. The viral MMTV promoter directed expression of Cre recombinase in the secretory epithelium of the mammary gland, the salivary gland and in the female germline. High levels of recombination have been detected in the virgin and lactating mammary gland, salivary gland, seminal vesicle, skin, and various cells of the immune system. Little background recombination was observed in the lung, kidney, liver and brain tissues. |
Mutations Made By | Dr. Kay-Uwe Wagner, University of Nebraska Medical Center |
When maintaining a live colony, heterozygous mice may be bred together or to wildtype mice from the colony.
When using the MMTV-Cre/Line A mouse strain in a publication, please cite the originating article(s) and include JAX stock #003551 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non Carrier for Tg(MMTV-cre)1Mam |
Frozen Mouse Embryo | STOCK Tg(MMTV-cre)1Mam/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(MMTV-cre)1Mam/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(MMTV-cre)1Mam/J | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(MMTV-cre)1Mam/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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