C57L/J-Tg(Tcrb)93Vbo/J

Stock number: 003540
Research areas: Research Tools

Description

These mice express a transgene containing a rearranged genomic segment derived from the Tcrb locus.

Detailed description

This transgenic mouse was constructed with a rearranged genomic segment derived from the Tcrb locus. T lymphocytes express the transgenic TCRB chain on cell surfaces while the protein products encoded by endogenous Tcrb genes are not expressed. Homozygous mice generally don't survive beyond postnatal week four. This strain may provide a specific developmental marker for a prothymocyte lineage subset that lacks pluripotential properties.

Development

The Tcrb genomic segment was constructed from B6 (Tcrb-V8.2,Tcrb-D) and BALB/c (Tcrb-J, Tcrb-C) sequences. The construct was injected into pronuclei from CBA/BrA X C57BL/LiA (F1)mice. To establish the line, a male founder was mated to a female C57L.

Allele

Symbol: Tg(Tcrb)93Vbo
Name: transgene insertion 93, Harald von Boehmer
MGI accession ID: MGI:3056727
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Tcrb)93Vbo
Marker name: transgene insertion 93, Harald von Boehmer
Chromosome: UN
Strain of origin: (CBA/BrA x C57BL/LiA)F2
Expressed genes: Tcrb,T cell receptor beta chain,mouse, laboratory
Molecular note: The transgene contains a rearranged genomic segment derived from the Tcrb locus, and was constructed from B6 (Tcrb-V8.2,Tcrb-D) and BALB/c (Tcrb-J, Tcrb-C) sequences. Over 98% of T cells express the transgenic TCR-beta chain in hemizygous mice. The rearranged beta-chain sequence was isolated from an anti-H-Y, H-2D^b-restricted CD8⁺ T cell clone, B6.2.16.
General note: In transgenic mice, T lymphocytes express the transgene on cell surfaces while the protein products encoded by endogenous Tcrb genes are not expressed. Most homozygous transgenic mice do not survive beyond postnatal week four.