immune system phenotype

• abnormal cytotoxic T cell physiology
  • female T cells bearing the transgenic TCR-beta chain respond to a 100-fold less HY antigen than transgenic male T cells do
  (MGI Ref ID J:66565)

• abnormal CD8-positive, alpha-beta T cell differentiation
  • 7-10% of CD8⁺ T cells from male mice recognize HY antigen in the context of H-2D^b though the affinity for antigen is 3-fold less
  (MGI Ref ID J:66565)
  • the reduced percentage and binding affinity is also evident in CD8 single-positive male thymocytes
  (MGI Ref ID J:66565)
  • 25-33% of peripheral CD8⁺ T cells from female mice recognize HY antigen in the context of H-2D^b compared to less than 1% of wild-type mice
  (MGI Ref ID J:66565)

• abnormal T cell clonal deletion
  • male mice have preferential deletion of thymocytes bearing TCR with transgenic beta chains that have high-affinity for HY antigen
  (MGI Ref ID J:66565)
  • the repertoire of T cell receptors (TCR) on T cells differs between male and female transgenic mice
  (MGI Ref ID J:66565)
  • HY specific male T cells represent the low-affinity fraction of the corresponding female T cell population
  (MGI Ref ID J:66565)

hematopoietic system phenotype

• abnormal CD8-positive, alpha-beta T cell differentiation
  • 7-10% of CD8⁺ T cells from male mice recognize HY antigen in the context of H-2D^b though the affinity for antigen is 3-fold less
  (MGI Ref ID J:66565)
  • 25-33% of peripheral CD8⁺ T cells from female mice recognize HY antigen in the context of H-2D^b compared to less than 1% of wild-type mice
  (MGI Ref ID J:66565)
  • the reduced percentage and binding affinity is also evident in CD8 single-positive male thymocytes
  (MGI Ref ID J:66565)

• abnormal T cell clonal deletion
  • HY specific male T cells represent the low-affinity fraction of the corresponding female T cell population
  (MGI Ref ID J:66565)
  • the repertoire of T cell receptors (TCR) on T cells differs between male and female transgenic mice
  (MGI Ref ID J:66565)
  • male mice have preferential deletion of thymocytes bearing TCR with transgenic beta chains that have high-affinity for HY antigen
  (MGI Ref ID J:66565)

• abnormal cytotoxic T cell physiology
  • female T cells bearing the transgenic TCR-beta chain respond to a 100-fold less HY antigen than transgenic male T cells do
  (MGI Ref ID J:66565)

hematopoietic system phenotype

• abnormal T cell receptor beta chain V(D)J recombination
  • this "partial" rearrangement occurs with high frequency and is not observed in wild-type T cells
  (MGI Ref ID J:93247)
  • peripheral T cells have rearranged Dbeta1-Jbeta segments that are not recombined with the Vbeta segments
  (MGI Ref ID J:93247)

• abnormal T cell morphology
  • Normal - cytolytic effector functions of T cells are not affected
  (MGI Ref ID J:93247)
  • almost all CD4⁺ and CD8⁺ T cells express the transgenic TCR-beta chain
  (MGI Ref ID J:93247)

immune system phenotype

• abnormal T cell receptor beta chain V(D)J recombination
  • this "partial" rearrangement occurs with high frequency and is not observed in wild-type T cells
  (MGI Ref ID J:93247)
  • peripheral T cells have rearranged Dbeta1-Jbeta segments that are not recombined with the Vbeta segments
  (MGI Ref ID J:93247)

• abnormal T cell morphology
  • almost all CD4⁺ and CD8⁺ T cells express the transgenic TCR-beta chain
  (MGI Ref ID J:93247)
  • Normal - cytolytic effector functions of T cells are not affected
  (MGI Ref ID J:93247)