These Kif3a knock-out mice exhibit randomized establishment of left-right asymmetry, display numerous structural abnormalities, and die at 10 days postcoitum. They are suitable for use in applications related to the study of asymmetry in development.
Joseph R. Marszalek, UCSD-HHMI
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Kif3a | kinesin family member 3A |
Ciliary formation appears to be crucial in developing left-right asymmetry in early mouse embryonic development. One of the necessary cilia components is the Kif3a gene product. Homozygous Kif3a knockout mice die at 10 days postcoitum, exhibit randomized establishment of left-right asymmetry and display numerous structural abnormalities. Cardiac looping is randomized, often retarded. Growth is severely retarded with caudal truncation. A neural tube closure defect is also apparent. Scanning electron microscopy indicates the absence of embryonic cilia. A small percentage of heterozygotes exhibit morphological abnormalities.
Allele Name | targeted mutation 1, Lawrence SB Goldstein |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | KIF3A-; Kif3an; Kif3atm1Maz |
Gene Symbol and Name | Kif3a, kinesin family member 3A |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 11 |
Molecular Note | Exon 2 was deleted by cre recombinase removal of a targeting vector containing the exon flanked by loxP sites. The resulting transcript was translated out of reading frame, generating a 62 amino acid nonsense protein. Northern and Western blots of heterozygous mutants showed that no fragments or aberrant products were made from the mutant gene. Western blots of homozygous mutants showed an absence of the targeted protein. This allele was generated from Kif3atm2Gsn. |
Mutations Made By | Joseph Marszalek, UCSD-HHMI |
Homozygotes are embryonic lethal (~embryonic day 10.5). When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
When using the KIF3A KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003537 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Kif3a<tm1Gsn> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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