These Kif3a knock-out mice exhibit randomized establishment of left-right asymmetry, display numerous structural abnormalities, and die at 10 days postcoitum. They are suitable for use in applications related to the study of asymmetry in development.
Joseph R. Marszalek, UCSD-HHMI
Ciliary formation appears to be crucial in developing left-right asymmetry in early mouse embryonic development. One of the necessary cilia components is the Kif3a gene product. Homozygous Kif3a knockout mice die at 10 days postcoitum, exhibit randomized establishment of left-right asymmetry and display numerous structural abnormalities. Cardiac looping is randomized, often retarded. Growth is severely retarded with caudal truncation. A neural tube closure defect is also apparent. Scanning electron microscopy indicates the absence of embryonic cilia. A small percentage of heterozygotes exhibit morphological abnormalities.
|Allele Name||targeted mutation 1, Lawrence SB Goldstein|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||KIF3A-; Kif3an; Kif3atm1Maz|
|Gene Symbol and Name||Kif3a, kinesin family member 3A|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Exon 2 was deleted by cre recombinase removal of a targeting vector containing the exon flanked by loxP sites. The resulting transcript was translated out of reading frame, generating a 62 amino acid nonsense protein. Northern and Western blots of heterozygous mutants showed that no fragments or aberrant products were made from the mutant gene. Western blots of homozygous mutants showed an absence of the targeted protein. This allele was generated from Kif3atm2Gsn.|
|Mutations Made By|| |
Joseph Marszalek, UCSD-HHMI
Homozygotes are embryonic lethal (~embryonic day 10.5). When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
When using the KIF3A KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003537 in your Materials and Methods section.