These Cdk knock-out mice exhibit unique lesions in the central nervous system associated with perinatal mortality. They are suitable for use in applications related to the study of brain development, neuronal differentiation, and neuronal cytoskeleton structure and organization.
Ashok B Kulkarni, NIDCR NIH
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cdk5 | cyclin-dependent kinase 5 |
Cdk5 is an important molecule for brain development and neuronal differentiation. Cdk null mice exhibit unique lesions in the central nervous system associated with perinatal mortality. The brains of mutant mice lack cortical laminar structure and cerebellar foliation. The large neurons in the brain stem and in the spinal cord show chromatolytic changes with accumulation of neurofilament immunoreactivity. They also exhibit neuronal migration abnormalities, cerebellar defoliation, NF accumulation neuronal bodies, and degenerated motor neurons.
Genomic clones of the mouse Cdk5 gene were isolated from a 129/SvJ mouse genomic library. Genomic fragments were used to construct the targeting vector, which carried a 0.8kb deletion including a part of exon III, and exons IV and V. The deletion was replaced by a neomycin resistance gene.
Allele Name | targeted mutation 1, Ashok B Kulkarni |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Cdk5 (-) |
Gene Symbol and Name | Cdk5, cyclin-dependent kinase 5 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 5 |
Molecular Note | Replacement of part of exon III, exons IV and V with a neomycin cassette. |
Mutations Made By | Ashok Kulkarni, NIDCR NIH |
The strain is on a mixed B6;129 background. Heterozygous mice appear normal; homozygous mice either die in utero or within 12 hours after birth. Expected coat color from breeding:Black,Agouti
When using the Cdk5- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003536 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Cdk5<tm1Kul> |
Frozen Mouse Embryo | B6;129-Cdk5<tm1Kul>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129-Cdk5<tm1Kul>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129-Cdk5<tm1Kul>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;129-Cdk5<tm1Kul>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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