B6;129-Cdk5^tm1Kul/J

Stock number: 003536
Product name: Cdk5-
Research areas: Developmental Biology Research, Neurobiology Research

Description

These Cdk knock-out mice exhibit unique lesions in the central nervous system associated with perinatal mortality. They are suitable for use in applications related to the study of brain development, neuronal differentiation, and neuronal cytoskeleton structure and organization.

Detailed description

Cdk5 is an important molecule for brain development and neuronal differentiation. Cdk null mice exhibit unique lesions in the central nervous system associated with perinatal mortality. The brains of mutant mice lack cortical laminar structure and cerebellar foliation. The large neurons in the brain stem and in the spinal cord show chromatolytic changes with accumulation of neurofilament immunoreactivity. They also exhibit neuronal migration abnormalities, cerebellar defoliation, NF accumulation neuronal bodies, and degenerated motor neurons.

Development

Genomic clones of the mouse Cdk5 gene were isolated from a 129/SvJ mouse genomic library. Genomic fragments were used to construct the targeting vector, which carried a 0.8kb deletion including a part of exon III, and exons IV and V. The deletion was replaced by a neomycin resistance gene.

Allele

Symbol: Cdk5^tm1Kul
Name: targeted mutation 1, Ashok B Kulkarni
MGI accession ID: MGI:1861809
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cdk5
Marker name: cyclin-dependent kinase 5
Chromosome: 5
Strain of origin: 129S4/SvJae
Molecular note: Replacement of part of exon III, exons IV and V with a neomycin cassette.