These Ltb knock-out mice exhibit a lack of Peyer's patches, peripheral lymph nodes, splenic germinal centers and follicular dendritic cells. They are suitable for use in applications related to the study of adaptive immune responses.
Dr. Richard A. Flavell, Yale University School of Medicine
The Ltb (lymphotoxin-beta) -deficient mice lack Peyer's patches, peripheral lymph nodes, splenic germinal centers and follicular dendritic cells. Note that this strain does have cervical and mesenteric lymph nodes, unlike Lta deficient mice. Mesenteric lymph nodes of Ltb deficient mice contain germinal center-like regions, but these areas appear to lack follicular dendritic cells. Homozygotes are viable and fertile.
A 0.7kb region of the Ltb gene is replaced with a neomycin cassette causing a truncation at codon 153, with numerous in-frame stop codons downstream of the neomycin cassette. A residual 23 codons of the Ltb protein-encoding sequence remain downstream of the neomycin resistance gene. The construct was electroporated into 129S1/Sv-derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J
|Allele Name||targeted mutation 1, Richard A Flavell|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||LT beta-; LTbeta-|
|Gene Symbol and Name||Ltb, lymphotoxin B|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||A neomycin resistance cassette replaced a 0.7 kb fragment of the gene, truncating the coding sequence at codon 153. Northern blots of total RNA from splenocytes from homozygous mutant mice showed an absence of mRNA for the targeted gene.|
|Mutations Made By|| |
Dr. Richard Flavell, Yale University School of Medicine
While maintaining a live colony, these mice are maintained by homozygote sibling matings.
When using the LTΒ KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003530 in your Materials and Methods section.