These Lrp8 knock-out mice exhibit a smaller and less foliated cerebellum with various hippocampus defects.
Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Lrp8 | low density lipoprotein receptor-related protein 8, apolipoprotein e receptor |
Mutant mice have a targeted mutation of the apolipoprotein E receptor Lrp8 (also called Apoer2). Homozygotes are viable with no gross morphological abnormalities. Homozygous males have reduced fertility, while females are not affected. Using C-terminal specific antibodies, no endogenous protein is detected in the brains of homozygotes. Homozygous mice have a smaller and less foliated cerebellum with various hippocampus defects in granule cell positioning, cortical neuron migration, granule cell laminar organization, commissural fiber distribution, CA1 microtubule-associated protein 2 (MAP-2) distribution, and long term potentiation (LTP). Mutant mice show contextual fear conditioning deficits. These mice may be useful in studies of brain development, neuronal cytoarchitecture, Reelin signaling pathways, NMDA receptor activity, lipoprotein receptors, synaptic plasticity and learning, schizophrenia, and neurodegenerative disorders such as Alzheimer's disease.
A targeting vector was designed to replace exons 17 and 18 of the endogenous gene with a pol2neo cassette. These two exons encode most of the membrane-spanning segment and a portion of the cytoplasmic tail. The construct was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Mutant mice were maintained on a mixed C57BL/6 and 129S6 background before arriving at The Jackson Laboratory.
Allele Name | targeted mutation 1, Joachim Herz |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | ApoER2-; Lp8tm1Her |
Gene Symbol and Name | Lrp8, low density lipoprotein receptor-related protein 8, apolipoprotein e receptor |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 4 |
Molecular Note | A neomycin resistance cassette replaced exons 17 and 18, which encode most of the membrane spanning segment and part of the cytoplasmic tail. |
Mutations Made By | Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas |
Homozygous males have reduced fertility.
When using the ApoER2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003524 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Lrp8<tm1Her> |
Frozen Mouse Embryo | B6;129S6-Lrp8<tm1Her>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S6-Lrp8<tm1Her>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S6-Lrp8<tm1Her>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;129S6-Lrp8<tm1Her>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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