B6;129S6-Lrp8^tm1Her/J

Stock number: 003524
Product name: ApoER2 KO
Research areas: Developmental Biology Research, Neurobiology Research, Research Tools

Description

These Lrp8 knock-out mice exhibit a smaller and less foliated cerebellum with various hippocampus defects.

Detailed description

Mutant mice have a targeted mutation of the apolipoprotein E receptor Lrp8 (also called Apoer2). Homozygotes are viable with no gross morphological abnormalities. Homozygous males have reduced fertility, while females are not affected. Using C-terminal specific antibodies, no endogenous protein is detected in the brains of homozygotes. Homozygous mice have a smaller and less foliated cerebellum with various hippocampus defects in granule cell positioning, cortical neuron migration, granule cell laminar organization, commissural fiber distribution, CA1 microtubule-associated protein 2 (MAP-2) distribution, and long term potentiation (LTP). Mutant mice show contextual fear conditioning deficits. These mice may be useful in studies of brain development, neuronal cytoarchitecture, Reelin signaling pathways, NMDA receptor activity, lipoprotein receptors, synaptic plasticity and learning, schizophrenia, and neurodegenerative disorders such as Alzheimer's disease.

Development

A targeting vector was designed to replace exons 17 and 18 of the endogenous gene with a pol2neo cassette. These two exons encode most of the membrane-spanning segment and a portion of the cytoplasmic tail. The construct was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Mutant mice were maintained on a mixed C57BL/6 and 129S6 background before arriving at The Jackson Laboratory.

Allele

Symbol: Lrp8^tm1Her
Name: targeted mutation 1, Joachim Herz
MGI accession ID: MGI:1861964
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Lrp8
Marker name: low density lipoprotein receptor-related protein 8, apolipoprotein e receptor
Chromosome: 4
Strain of origin: 129S6/SvEvTac
Molecular note: A neomycin resistance cassette replaced exons 17 and 18, which encode most of the membrane spanning segment and part of the cytoplasmic tail.