C57BL/6J-Tg(SV)427Bri/J

Stock number: 003476
Product name: 427-line
Research areas: Cancer Research

Description

These 427-line mice express a transgene containing SV40 T antigen early region genes. They show a high incidence of brain choroid plexus tumors and are unresponsive to immunization with SV40antigen.

Detailed description

This 427 transgenic strain and the 419 strain C57BL/6J-Tg(SV)427Bri/J (Stock No. 003477) each contain the same SV40 T antigen early region genes in the SVMK plasmid construct, but differ in choroid plexus tumor incidence and immune response to SV40 T antigen. Strain 427 mice developed 95 tumors out of 96 mice while 419 mice developed 1 tumor out of 53 mice. Strain 427 mice are completely unresponsive to immunization with SV40 while mice mount a normal immune response to SV40 T antigen compared with non-transgenic control mice. (Faas SJ, Pan S, Pinkert CA, Brinster RL, Knowles BB. Simian virus 40 (SV40)-transgenic mice that develop tumors are specifically tolerant to SV40 T antigen. J Exp Med. 1987 Feb 1;165(2):417-27.)

Transgenic males are sterile.

Development

The transgene, containing the SVMK plasmid consisting of a pSV3-ts58-derived Kpn I-Bam HI fragment, was injected into the pronuclei of C57BL/6J mouse oocytes. Mice from founder line 427 bred with C57BL/6J mice to establish the colony.

Allele

Symbol: Tg(SV)427Bri
Name: transgene insertion 427, Ralph L Brinster
MGI accession ID: MGI:3056377
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(SV)427Bri
Marker name: transgene insertion 427, Ralph L Brinster
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: TAg,SV40 large T-antigen,SV40
Molecular note: The transgene contains the SV40 enhancer and large-T antigen.