These Tcrd knock-out mice exhibit deficiency of gamma delta T-cell receptor expression.
Read More +Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Tcrd | T cell receptor delta chain |
Mice homozygous for this targeted mutation are viable and fertile. Gamma delta T-cell receptor expression is deficient in all adult lymphoid and epithelial organs. There is normal development of the alpha beta T-cell lineage. Patterns of CD4+CD8- and CD4-CD8+ alpha beta T-cells are apparently normal. Mice do not develop inflammatory bowel disease.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
This targeted mutant strain was developed by Dr. Peter Mombaerts in the laboratory of Dr. Susumu Tonegawa at the Center for Cancer Research, Massachusetts Institute of Technology. A replacement targeting vector containing Pgk-neo was used which resulted in a 4 kb deletion encompassing most of the delta chain constant region coding sequences. This strain was generated in the laboratory of Dr. Leonard Shultz by backcrossing mice from B6.1292-Tcrdtm1Mom/J to BALB/cByJ inbred mice for a minimum of 9 generations.
Allele Name | targeted mutation 1, Peter Mombaerts |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | C-delta-; delta-; delta0; gammadelta TCR-; TCRdelta-; TCRdelta KO |
Gene Symbol and Name | Tcrd, T cell receptor delta chain |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 14 |
Molecular Note | A PGK-neo cassette was used to disrupt the constant gene segment (designated C-delta). Flow cytometry showed that cells isolated from the lymphoid or epithelial organs of homozygous mice did not react with an anti-pan Tcrd mAb. Immunoprecipitation showed an absence of expression on the surface of thymocytes isolated from homozygous mutant embryos. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
When using the BALB/cBy TCRdelta- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003448 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Tcrd<tm1Mom> |
Frozen Mouse Embryo | CByJ.129P2(B6)-Tcrd<tm1Mom>/Sz | $2595.00 |
Frozen Mouse Embryo | CByJ.129P2(B6)-Tcrd<tm1Mom>/Sz | $2595.00 |
Frozen Mouse Embryo | CByJ.129P2(B6)-Tcrd<tm1Mom>/Sz | $3373.50 |
Frozen Mouse Embryo | CByJ.129P2(B6)-Tcrd<tm1Mom>/Sz | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.