These mice carry a spontaneous mutation at the Pepd locus characterized by a darkened coat color, smaller size, gonad abnormalities, and dark staining urine.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous (Hypomorph) | Pepd | peptidase D |
Dark-like dal is a recessive mutation causing a darkened coat color, smaller size, gonad abnormalities, and dark staining urine. dal maps to Chromosome 7 and a previously described mutation named dark da maps to the same chromosomal region. Dark-like may be a remutation to dark da.However a test for allelism is not possible because dark is thought to be extinct. Mice homozygous for the dal mutation are easily recognized by 14 days of age by their darkened coats and smaller size. Some homozygotes appear, both phenotypically and pathologically (dense bones), to have skeletal abnormalities however X-rays appear normal. At 7 months of age females had no follicles and many corpora lutea and males mild testicular degeneration with increased Leydig cells. Serum assays for Albumin, BUN, Creatine, Bilirubin, and iron showed no significant differences from controls.
dal arose spontaneously in 1981 in the CBA/J colony of Dr. Eva Eicher at The Jackson Laboratory. The CBA/J colony was then at F182. This strain has been maintained via sibling mating and in 2004 reached F182+F72. Embryos were generated for cryopreservation using F182+52+ homozygous males bred with CBA/J females.
Allele Name | dark-like |
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Allele Type | Spontaneous (Hypomorph) |
Allele Synonym(s) | |
Gene Symbol and Name | Pepd, peptidase D |
Gene Synonym(s) | |
Strain of Origin | CBA/J |
Chromosome | 7 |
Molecular Note | This spontaneous mutation is a 4 base pair deletion of ACTG in exon 14 that is predicted to cause a frameshift after amino acid 409 and replace the 84 carboxy terminal amino acids with 45 novel amino acids. QPCR shows a 42% decrease in expression in homozygous embryonic hearts and prolidase activity is reduced to undetectable levels in adult liver, E15.5 and adult heart, and adult red blood cells. |
When using the dark-like mouse strain in a publication, please cite the originating article(s) and include JAX stock #003398 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for dal |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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