129S-Nog^tm1Amc/J

Stock number: 003383
Product name: Noggin KO
Research areas: Developmental Biology Research, Neurobiology Research, Research Tools

Description

These Nog knock-out, lacZ reporter mice exhibit multiple defects, including an open neural tube, skeletal abnormalities, shortened body axis, and a small vestigial tail.

Detailed description

Homozygous mice are born but die shortly after birth, exhibiting multiple defects, including an open neural tube, skeletal abnormalities, shortened body axis, and a small vestigial tail. Analysis of early gene expression has shown that the loss of Nog expression in the floorplate, notochord, and roofplate results in a progressive failure of ventral development in the CNS and somites. Nog is also expressed in condensing cartilage in the limb and in the sclerotome of somites so its loss results in defects in cartilage patterning and skeletal morphogenesis. Heterozygous embryos show lacZ reporter expression in pattern consistent with the endogenous gene.

Development

A targeting vector containing neomycin resistance, herpes simplex virus thymidine kinase and lacZ genes was used to disrupt most of the Nog coding region and some 3' flanking sequence. The targeted allele consists of an in frame lacZ fusion to noggin's first 10 amino acids. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were backcrossed to 129/Sv mice.

Allele

Symbol: Nog^tm1Amc
Name: targeted mutation 1, Andrew P McMahon
MGI accession ID: MGI:1862000
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Nog
Marker name: noggin
Chromosome: 11
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: Expression of the lacZ reporter is consistent with the expression pattern of the endogenous gene.
Molecular note: Gene targeting generated a null allele by fusing the first 10 amino acids of coding sequence in-frame to the lacZ gene. The remainder of the coding sequence and some of the 3' flanking sequence of the gene were deleted. Ectopic expression of the resulting transcript was noted.