| Allele Name | transgene insertion 3, David R Borchelt |
| Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
| Allele Synonym(s) | APP695; APP695swe; APPswe; line C3-3; Mo/HuAPPswe |
| Gene Symbol and Name | Tg(APP695)3Dbo, transgene insertion 3, David R Borchelt |
| Gene Synonym(s) | |
| Promoter | Prn, prion protein readthrough transcript, mouse, laboratory |
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| Expressed Gene | APP695, amyloid beta (A4) precursor protein (chimeric), mouse/human chimera |
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| Strain of Origin | (C57BL/6J x C3H/HeJ)F2 |
| Chromosome | UN |
| General Note | Three transgenic lines were generated and designated by the authors lines Q2-2, E1-2 (Tg(Prnp-App/APPswe)E1-2Dbo) and C3-3. This line was generated from founder number C3-3. Transgenic mice develop amyloid deposits in brain tissue by 18-20 months of age. Transgenic mice that are also transgenic for Tg(PSEN1)5Dboexpress both human presenilin 1 (A246E variant) and a chimeric amyloid precursor protein (APPSwe) under direction of the mouse prion protein promoter. Elevated levels of the AB1-42(43) peptide are detected in brain homogenates. By nine months of age, histological examination of brain tissue from these mice reveals numerous amyloid deposits resembling those observed in the brains of patients with Alzheimer's disease (AD). The number of amyloid deposits increases dramatically between the ages of 10 and 12 months. |
| Molecular Note | The transgene is composed of a cDNA encoding a chimeric APP protein regulated by the mouse prion promoter. The chimeric APP molecule was created by replacing sequences encoding the Abeta domain of a 695 amino acid isoform of the murine sequence with the cognate sequences of the human gene (mutations K595N, M596L). The human mutations are found in familial Alzheimer's disease. Transgene expression was observed in the brain and heart by Western blot analysis using a monoclonal antibody recognizing the human Abeta region. |
| Mutations Made By | Dr. David Borchelt, University of Florida |
