These Cux1 knock-out mice express a mutant form of the Cutl1 protein with a deletion of the first DNA binding domain, and exhibit curly vibrissae and wavy hair.
Dr. Ellis J. Neufeld, Harvard Medical School
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cux1 | cut-like homeobox 1 |
CDP, a ubiquitous homeoprotein homologous to Drosophila cut, is implicated as a transcriptional repressor in several developmental systems. It contains four independent DNA binding domains: three "cut repeats" plus the homeodomain. The mouse Cux/CDP gene spans more than 200 kb and is composed of at least 21 exons. B6;129-Cutl1tm1Ejn mice express a mutant form of the Cutl1 protein. Homozygotes display curly vibrissae and wavy hair. There is a high degree of pup loss in litters born to mutant females. The mutant protein is present at levels slightly greater than wild-type, but exhibits the same tissue distribution as wild-type protein, and has approximately normal affinity for known target sequences.
Allele Name | targeted mutation 1, Ellis J Neufeld |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | deltaCR1 |
Gene Symbol and Name | Cux1, cut-like homeobox 1 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 5 |
Molecular Note | A neomycin resistance cassette replaced a genomic fragment containing exons 10 and 11. Western blot analysis on spleen and thymus extracts of homozygous mice demonstrated that a mutant protein that lacked cut domain 1, but retains DNA binding activity, was produced from this allele. |
Mutations Made By | Dr. Ellis Neufeld, Harvard Medical School |
The donating investigator maintained the strain by using heterozygous matings. There is a reduced viability of pups born to null females - but normal viability of pups (regardless of genotype) born to heterozygous females mated to homozygous or heterozygous males. Reproduction is fair to good for heterozygotes. Homozygotes are viable and fertile. The expected coat color from breeding is Black, White Bellied Agouti.
When using the ΔCR1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #003366 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Cux1<tm1Ejn> |
Frozen Mouse Embryo | B6;129S4-Cux1<tm1Ejn>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Cux1<tm1Ejn>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Cux1<tm1Ejn>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S4-Cux1<tm1Ejn>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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