B6;129S4-Cux1^tm1Ejn/J

Stock number: 003366
Product name: ΔCR1
Research areas: Dermatology Research

Description

These Cux1 knock-out mice express a mutant form of the Cutl1 protein with a deletion of the first DNA binding domain, and exhibit curly vibrissae and wavy hair.

Detailed description

CDP, a ubiquitous homeoprotein homologous to Drosophila cut, is implicated as a transcriptional repressor in several developmental systems. It contains four independent DNA binding domains: three "cut repeats" plus the homeodomain. The mouse Cux/CDP gene spans more than 200 kb and is composed of at least 21 exons. B6;129-Cutl1^tm1Ejn mice express a mutant form of the Cutl1 protein. Homozygotes display curly vibrissae and wavy hair. There is a high degree of pup loss in litters born to mutant females. The mutant protein is present at levels slightly greater than wild-type, but exhibits the same tissue distribution as wild-type protein, and has approximately normal affinity for known target sequences.

Allele

Symbol: Cux1^tm1Ejn
Name: targeted mutation 1, Ellis J Neufeld
MGI accession ID: MGI:1861916
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cux1
Marker name: cut-like homeobox 1
Chromosome: 5
Strain of origin: 129S4/SvJae
Molecular note: A neomycin resistance cassette replaced a genomic fragment containing exons 10 and 11. Western blot analysis on spleen and thymus extracts of homozygous mice demonstrated that a mutant protein that lacked cut domain 1, but retains DNA binding activity, was produced from this allele.