These Cux1 knock-out mice express a mutant form of the Cutl1 protein with a deletion of the first DNA binding domain, and exhibit curly vibrissae and wavy hair.
Dr. Ellis J. Neufeld, Harvard Medical School
CDP, a ubiquitous homeoprotein homologous to Drosophila cut, is implicated as a transcriptional repressor in several developmental systems. It contains four independent DNA binding domains: three "cut repeats" plus the homeodomain. The mouse Cux/CDP gene spans more than 200 kb and is composed of at least 21 exons. B6;129-Cutl1tm1Ejn mice express a mutant form of the Cutl1 protein. Homozygotes display curly vibrissae and wavy hair. There is a high degree of pup loss in litters born to mutant females. The mutant protein is present at levels slightly greater than wild-type, but exhibits the same tissue distribution as wild-type protein, and has approximately normal affinity for known target sequences.
|Allele Name||targeted mutation 1, Ellis J Neufeld|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Cux1, cut-like homeobox 1|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A neomycin resistance cassette replaced a genomic fragment containing exons 10 and 11. Western blot analysis on spleen and thymus extracts of homozygous mice demonstrated that a mutant protein that lacked cut domain 1, but retains DNA binding activity, was produced from this allele.|
|Mutations Made By|| |
Dr. Ellis Neufeld, Harvard Medical School
The donating investigator maintained the strain by using heterozygous matings. There is a reduced viability of pups born to null females - but normal viability of pups (regardless of genotype) born to heterozygous females mated to homozygous or heterozygous males. Reproduction is fair to good for heterozygotes. Homozygotes are viable and fertile. The expected coat color from breeding is Black, White Bellied Agouti.
When using the ΔCR1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #003366 in your Materials and Methods section.