These Jup knock-out mice exhibit embryonic or early death with skin blistering and subcorneal acantholysis.
Prof. Dr. RolfK Kemler, Max Planck Institute for Immunobiology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Jup | junction plakoglobin |
Plakoglobin Jup null-mutant embryos die from embryonic day 10.5 onward due to severe heart defects. Some mutant embryos develop further, especially on a C57BL/6 background. These mice die around birth, presumably due to cardiac dysfunction, and with skin blistering and subcorneal acantholysis. The skin phenotype in plakoglobin-deficient mice is reminiscent of the human blistering disease, epidermolytic hyperkeratosis.
A targeting vector containing the neomycin resistance cassette was used to replace exons 3 and 4. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were bred to crossed to C57BL/6J and offspring were backcrossed to C57BL/6J for at least 7 generations.
Allele Name | targeted mutation 1, Rolf Kemler |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Pg-; plako- |
Gene Symbol and Name | Jup, junction plakoglobin |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 11 |
Molecular Note | A neomycin resistance cassette replaced exons 3 and 4 of the gene. |
Mutations Made By | Prof. Dr. RolfK Kemler, Max Planck Institute for Immunobiology |
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony. Mice homozygous for the mutation are not viable, however, a few plakoglobin null mutant embryos may make it to birth, but usually do not survive.
When using the plako- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003360 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Jup<tm1Kem> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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