These En1 knock-out mice exhibit postnatal death with missing third and fourth cranial nerves as well as most of the colliculi and cerebellum. They are suitable for use in applications related to the study of patterning of the central nervous system during vertebrate development.
IMR Colony, The Jackson Laboratory
Mice homozygous for the En1tm1Alj targeted mutation die shortly after birth. They are missing the third and fourth cranial nerves as well as most of the colliculi and cerebellum. The brain phenotype can be less severe depending on the genetic background. There is also a disruption of the dorsal/ventral patterning of the limb paws, a disrupted sterum, and truncation of the 13th ribs. Deletion of mid-hindbrain tissue may be seen as early as embryonic day 9.5.
|Allele Name||targeted mutation 1, Alexandra L Joyner|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||En1, engrailed 1|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A neomycin selection cassette replaced 0.85 kb of exon 2, which encodes part of the homeobox domain.|
|Mutations Made By|| |
Dr. Alexandra Joyner, Memorial Sloan-Kettering Cancer Center
Homozygotes die shortly after birth. When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
When using the En-1hd mouse strain in a publication, please cite the originating article(s) and include JAX stock #003343 in your Materials and Methods section.