These IκBα(ΔN) mice express a transgene containing an amino acid-truncated human NFKBIA gene fused to a FLAG epitope under the control of a human LCK promoter and are useful in studies of T-cell development.
Dr. Dean W. Ballard, Vanderbilt University School of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Dominant negative, Inserted expressed sequence, Humanized sequence) |
Mice hemizygous for TgN(LCK-NFKBIA)5Dwb transgene exhibit a complete inactivation of the NFKB signaling pathway. The T cell lineage expresses a trans-dominant form of IkBa that constitutively represses the activity of multiple NFKB/Rel proteins. Transgenic cells expressing this inhibitor exhibit a significant proliferative defect. Mitogenic stimulation of splenocytes leads to increased apoptosis of transgenic T cells. Transgene expression also impairs the development of normal T cell populations as evidenced by diminished numbers of TCR hiCD8 single-positive thymocytes. This defect is significantly amplified in the periphery and is accompanied by a decrease in CD4 + T cells.
Expressed Gene | NFKBIA, NFKB inhibitor alpha, human |
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Site of Expression |
Allele Name | transgene insertion 5, Dean W Ballard |
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Allele Type | Transgenic (Dominant negative, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | IkappaBalpha(deltaN); IkappaNalphadeltaN-Tg; LCK |
Gene Symbol and Name | Tg(LCK-NFKBIA)5Dwb, transgene insertion 5, Dean W Ballard |
Gene Synonym(s) | |
Promoter | LCK, LCK proto-oncogene, Src family tyrosine kinase, human |
Expressed Gene | NFKBIA, NFKB inhibitor alpha, human |
Strain of Origin | C57BL/6 x DBA/2 |
Chromosome | UN |
Molecular Note | An amino acid-truncated human NFKBIA gene (coding for amino acids 37-317) fused to a FLAG epitope was placed under the control of a human LCK promoter. The protein encoded by this transgene lacks sequences required for signal-dependent degradation and functions as a constitutive repressor of multiple NK-kappaB pathways. Transgene expression was readily detected in the thymus and spleen as determined by immunoblot analysis. |
Mutations Made By | Dr. Dean Ballard, Vanderbilt University School of Medicine |
When maintaining a live colony, homozygous mice may be bred together.
When using the IκBα(ΔN) mouse strain in a publication, please cite the originating article(s) and include JAX stock #003330 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(LCK-NFKBIA)5Dwb |
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