Mice hemizygous for TgN(LCK-NFKBIA)5Dwb transgene exhibit a complete inactivation of the NFKB signaling pathway. The T cell lineage expresses a trans-dominant form of IkBa that constitutively represses the activity of multiple NFKB/Rel proteins. Transgenic cells expressing this inhibitor exhibit a significant proliferative defect. Mitogenic stimulation of splenocytes leads to increased apoptosis of transgenic T cells. Transgene expression also impairs the development of normal T cell populations as evidenced by diminished numbers of TCR <sup>hi</sup>CD8 single-positive thymocytes. This defect is significantly amplified in the periphery and is accompanied by a decrease in CD4 <sup>+</sup> T cells.

These I&kappa;B&alpha;(&Delta;N) mice express a transgene containing an amino acid-truncated human NFKBIA gene fused to a FLAG epitope under the control of a human LCK promoter and are useful in studies of T-cell development.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

<a target="_blank" href="https://www.jax.org/jax-mice-and-services/customer-support" rel="noreferrer">Contact Customer Service</a> for more information.