These Shh knock-out mice exhibit defects in the establishment and maintenance of midline structures.
Andrew P McMahon, University of Southern California
Mice homozygous for the Shhtm1Amc targeted mutation display early defects in the establishment and maintenance of midline structures. These defects result from the critical role the Sonic hedgehog (Shh) gene plays in the patterning of vertebrate embryonic tissues, including the brain and spinal cord, the axial skeleton and the limbs. Defects are also observed in all tissues, confirming the proposed role of SHH proteins as an extracellular signal required for the tissue-organizing properties of several vertebrate patterning centers.
When bred to a strain with loxP sites inserted into the same targeted allele (Stock No. 004293) and a strain expressing Cre recombinase in the skin and dental epithelium (Stock No. 004782), this mutant mouse strain may be useful in studies of hedgehog signalling and cell proliferation in the dental epithelium.
When bred to a strain with loxP sites inserted into the same targeted allele (Stock No. 004293) and a strain expressing Cre recombinase in the mesonephric duct and its developmental derivatives (Stock No. 004692), this mutant mouse strain may be useful in studies of hedgehog signaling and cell proliferation/differentiation in mesenchymal cells of the kidney.
|Allele Name||targeted mutation 1, Andrew P McMahon|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Shh-; Shhn|
|Gene Symbol and Name||Shh, sonic hedgehog|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A neomycin cassette replaced exon 2 and portions of the flanking intronic sequences.|
|Mutations Made By|| |
Dr. Paula Lewis, AstraZeneca, R&D Boston
Homozygotes are not viable. The strain is maintained by mating heterozygotes. The expected coat color from breeding is Black, White Bellied Agouti.
When using the Shh- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003318 in your Materials and Methods section.