B6.129P2-Gck^tm1Efr/J

Stock number: 003316
Product name: GK-
Research areas: Developmental Biology Research, Diabetes and Obesity Research, Endocrine Deficiency Research

Description

These Gck knock-out mice exhibit a decreased insulin secretory response to glucose. They are suitable for use in applications related to the study of maturity-onset diabetes.

Detailed description

Glucokinase catalyzes a rate-limiting step in glucose metabolism in hepatocytes and pancreatic beta cells and is considered the "glucose sensor" for regulation of insulin secretion. Patients with maturity-onset diabetes of the young (MODY) have heterozygous point mutations in the glucokinase gene that result in reduced enzymatic activity and decreased insulin secretion. Disruption of the glucokinase gene results in a phenotype similar to MODY in heterozygous mice. These mice manifest a decreased insulin secretory response to glucose.

Allele

Symbol: Gck^tm1Efr
Name: targeted mutation 1, Shimon Efrat
MGI accession ID: MGI:1861929
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Gck
Marker name: glucokinase
Chromosome: 11
Strain of origin: 129P2/OlaHsd
Molecular note: A fragment of the gene spanning exon 2 was replaced by a neomycin resistance cassette, resulting in a deletion and a frame shift of the transcript. Mice heterozygous for the targeted mutation had a 37% reduction in glucose phosphorylation activity in islet homogenates and a 28% reduction of activity in liver compared with wild-type littermates.