This line carries a Cre transgene under the control of the adenovirus EIIa promoter that targets expression of Cre recombinase to the early mouse embryo. Cre-mediated recombination occurs in a wide range of tissues, including the germ cells that transmit the genetic alteration to progeny.
Dr. Heiner Westphal, National Institutes of Health
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Recombinase-expressing) |
This line carries a Cre transgene under the control of the adenovirus EIIa promoter that targets expression of Cre recombinase to the early mouse embryo. Cre expression is thought to occur prior to implantation in the uterine wall. A mosaic pattern of expression is commonly observed. Cre-mediated recombination occurs in a wide range of tissues, including the germ cells that transmit the genetic alteration to progeny.
View cre expression characterization.
The EIIa-Cre plasmid was constructed by replacing the human cytomegalovirus promoter in pBS185 with the adenovirus EIIa promoter from pEII-lacZ. cre activity present in zygotes of the EIIa-cre genotype was measured as follows. EIIa-cre/EIIa-cre homozygous animals were mated with +/alphaA-loxP-STOP-loxP-TAg heterozygous animals and the presence of lens tumors in double transgenic progeny, indicative of cre-mediated excision of STOP sequences and a concomitant activation of SV40 TAg, was scored. The strain originated on an FVB/N background. The strain is homozygous for the transgene. Analysis at The Jackson Laboratory Repository suggests that the transgene has integrated into a region on chromosome 6 between 18 Mbp and 95 Mbp (see additional information below).
Some FVB.EIIa-Cre mice from this colony were used for marker-assisted backcrossing onto other inbred backgrounds. During that process, single nucleotide polymorphism (SNP) testing revealed a region on chromosome 6 between 18 Mbp and 95 Mbp that remained FVB allele-type in EIIa-Cre transgenic mice. These data suggest the transgene has integrated into this region of chromosome 6.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | widespread expression (including germline); mosaic pattern commonly observed |
Allele Name | transgene insertion C5379, Laboratory of Mammalian Genes and Development, Heiner Westphal |
---|---|
Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | E2a-Cre; EIIa Cretg; EIIaCre; EIIa-cre; EIIaCre deleter; EII-cre; Ela-Cre; Ella:Cre; TgN(EIIa-Cre)C5379Lmgd |
Gene Symbol and Name | Tg(EIIa-cre)C5379Lmgd, transgene insertion C5379, Laboratory of Mammalian Genes and Development, Heiner Westphal |
Gene Synonym(s) | |
Promoter | Ella, adenovirus, adenovirus |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | widespread expression (including germline); mosaic pattern commonly observed |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The adenovirus EIIa promoter directs expression of Cre recombinase in preimplantation mouse embryos. This transgene expresses Cre recombinase in nearly all tissues. |
Mutations Made By | Dr. Heiner Westphal, National Institutes of Health |
When maintaining a live colony, these mice are bred by homozygote sibling matings. The expected coat color from breeding is albino.
When using the E2a-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #003314 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non-carrier for Tg(EIIa-cre)C5379Lmgd |
Frozen Mouse Embryo | FVB/N-Tg(EIIa-cre)C5379Lmgd/J | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(EIIa-cre)C5379Lmgd/J | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(EIIa-cre)C5379Lmgd/J | $3373.50 |
Frozen Mouse Embryo | FVB/N-Tg(EIIa-cre)C5379Lmgd/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.