FVB/N-Tg(EIIa-cre)C5379Lmgd/J

Stock number: 003314
Product name: E2a-Cre
Research areas: Research Tools

Description

This line carries a Cre transgene under the control of the adenovirus EIIa promoter that targets expression of Cre recombinase to the early mouse embryo. Cre-mediated recombination occurs in a wide range of tissues, including the germ cells that transmit the genetic alteration to progeny.

Detailed description

This line carries a Cre transgene under the control of the adenovirus EIIa promoter that targets expression of Cre recombinase to the early mouse embryo. Cre expression is thought to occur prior to implantation in the uterine wall. A mosaic pattern of expression is commonly observed. Cre-mediated recombination occurs in a wide range of tissues, including the germ cells that transmit the genetic alteration to progeny.

View cre expression characterization.

Development

The EIIa-Cre plasmid was constructed by replacing the human cytomegalovirus promoter in pBS185 with the adenovirus EIIa promoter from pEII-lacZ. cre activity present in zygotes of the EIIa-cre genotype was measured as follows. EIIa-cre/EIIa-cre homozygous animals were mated with +/alphaA-loxP-STOP-loxP-TAg heterozygous animals and the presence of lens tumors in double transgenic progeny, indicative of cre-mediated excision of STOP sequences and a concomitant activation of SV40 TAg, was scored. The strain originated on an FVB/N background. The strain is homozygous for the transgene. Analysis at The Jackson Laboratory Repository suggests that the transgene has integrated into a region on chromosome 6 between 18 Mbp and 95 Mbp (see additional information below).

Some FVB.EIIa-Cre mice from this colony were used for marker-assisted backcrossing onto other inbred backgrounds. During that process, single nucleotide polymorphism (SNP) testing revealed a region on chromosome 6 between 18 Mbp and 95 Mbp that remained FVB allele-type in EIIa-Cre transgenic mice. These data suggest the transgene has integrated into this region of chromosome 6.

Allele

Symbol: Tg(EIIa-cre)C5379Lmgd
Name: transgene insertion C5379, Laboratory of Mammalian Genes and Development, Heiner Westphal
MGI accession ID: MGI:2137691
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(EIIa-cre)C5379Lmgd
Marker name: transgene insertion C5379, Laboratory of Mammalian Genes and Development, Heiner Westphal
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: widespread expression (including germline); mosaic pattern commonly observed
Molecular note: The adenovirus EIIa promoter directs expression of Cre recombinase in preimplantation mouse embryos. This transgene expresses Cre recombinase in nearly all tissues.