These Ngf knock-out mice exhibit short life spans with delayed development and cell loss in sympathetic ganglia. They are suitable for use in studies related to the effect of nerve growth factor on neuron development.
Dr. Heidi Phillips, Genentech, Inc.
Mice homozygous for the disrupted Ngfb gene are born alive, but are smaller, on average, than wild type or heterozygous individuals. They fail to thrive and have a life span of 4 weeks or less, often dying within the first three days of life. Mutant mice are developmentally delayed and exhibit severe cell loss in sympathetic ganglia. They exhibit a more selective cell loss in sensory ganglia, revealing a reduced number of small cells in the dorsal root ganglia (DRG) at 3 days of age, while large cell numbers in the DRG are comparable to wild type mice. Mutant mice also display a decreased responsiveness to pain in comparison to +/+ or +/- littermates. During the first month of life, survival of cholinergic neurons in the basal forebrain does not appear to be affected by absence of NGF, as these cells were observed to differentiate and maintain their phenotype throughout the life span of homozygous mutant mice. Mice heterozygous for the Ngfb gene disruption exhibit normal growth and development and breed normally.
|Allele Name||targeted mutation 1, Genentech|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||NGF-; Ngfbtm1|
|Gene Symbol and Name||Ngf, nerve growth factor|
|Strain of Origin||129S7/SvEvBrd-Hprt+|
|Molecular Note||Replacement of a 150 bp fragment of the Ngfb gene, including the exon IV splice acceptor site and 130-bp of NGF coding sequence including the translation start site, with a CMV-neomycin cassette.|
3/31/00: per Debbie Mayo: B6 x +/- or +/- x B6 The investigator maintains the strain by backcrossing heterozygotes to C57BL/6. Homozygotes die by 4 weeks of age.
When using the NGF KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003312 in your Materials and Methods section.