129S6/SvEvTac-Was^tm1Sbs/J

Stock number: 003292
Product name: WASP-
Research areas: Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Mouse/Human Gene Homologs

Description

These Was knock-out mice exhibit reduced peripheral blood lymphocyte counts and platelet numbers along with development of chronic colitis. They are suitable for use in applications related to the study of Wiskott-Aldrich syndrome in humans.

Detailed description

WAS-deficient mice are viable and fertile. Mutant mice show normal lymphocyte development, serum immunoglobulin (Ig) levels and antibody responses. However, peripheral blood lymphocyte counts and platelet numbers are reduced in these mice. Development of chronic colitis is also observed. In vitro, WAS-deficient T cells show markedly impaired proliferative responses to anti-CD3e mediated stimulation. The Was gene is X-linked, so hemizygous males are WAS deficient.

Allele

Symbol: Was^tm1Sbs
Name: targeted mutation 1, Scott B Snapper
MGI accession ID: MGI:1861707
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Was
Marker name: Wiskott-Aldrich syndrome
Chromosome: X
Strain of origin: 129S6/SvEvTac
Molecular note: A neomycin cassette was inserted into exon 7, which encodes part of the GTPase-binding domain. The absence of transcript and protein produced from the targeted allele in male hemizygous mutant mice was confirmed by Northern blot and Western blot analyses.