STOCK Tg(Trp53A135V)L3Ber/J

Stock number: 003262
Product name: PL53-3
Research areas: Cancer Research, Internal/Organ Research, Sensorineural Research

Detailed description

Mice homozygous for the (Trp53A135V)2Ber transgene are viable and fertile, but show a high incidence of tumors, particularly lung adenocarcinomas, osteosarcomas, and lymphomas. This strain may serve as a model for Li-Fraumeni syndrome.

Development

This strain was developed on a CD1 genetic background.

Allele

Symbol: Tg(Trp53A135V)L3Ber
Name: transgene insertion L3, Alan Bernstein
MGI accession ID: MGI:2447408
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: pL53-3; Tg(pL53)3Ber; Tg(Trp53A135V)2Ber
Marker symbol: Tg(Trp53A135V)L3Ber
Marker name: transgene insertion L3, Alan Bernstein
Marker synonyms: p53^val135; pL53-3; Tg(pL53)3Ber; Tg(Trp53A135V)2Ber; UL53-3
Chromosome: UN
Strain of origin: CD-1
Expressed genes: Trp53,transformation related protein 53,mouse, laboratory
Molecular note: The transgene contains the mouse transformation related protein 53 promoter and an allele of the mouse Trp53 gene that carries a point mutation in codon 135 (GCG to GTG), resulting in an alanine to valine substitution. The Trp53 promoter drove high levels of transgenic expression in the spleen, thymus, and lymph nodes. Transgenic expression was also reported in the ovaries. Little or no expression was reported to have been observed in the liver and lungs of these mice.
General note: Additional lines L1 and L2 were produced.

Homozygous transgenic mice are viable and fertile, but show a high incidence of tumors, particularly lung adenocarcinomas, osteosarcomas, and lymphomas.

Allele

Symbol: Gnat2^cpfl3
Name: cone photoreceptor function loss 3
MGI accession ID: MGI:3588845
Generation method: Spontaneous
Synonyms: no cones
Marker symbol: Gnat2
Marker name: guanine nucleotide binding protein, alpha transducing 2
Marker synonyms: ACHM4; Gnat-2; GNATC; Gt-2; HG1D; Tcalpha
Chromosome: 3
Strain of origin: various
Molecular note: A single nucleotide substitution of G to A at coding nucleotide 598 in exon 6. This mutation converts codon 200 from aspartic acid to asparagine (p.D200N).
General note: This allele has been detected in the following strains either by genotyping or complementation testing: ALS/LtJ, SENCARA/PtJ, SENCARB/PtJ, SENCARC/PtJ, PN/nBSwUmabJ. (J:122428) The allele has also been reported in NMRI and CD1 (Lluis Montoliu, J:212307)