B6.129S7-Tnfrsf1b^tm1Imx/J

Stock number: 003246
Product name: p75-
Research areas: Apoptosis Research, Cancer Research, Immunology, Inflammation and Autoimmunity Research

Description

These Tnfrsf1b knock-out mice exhibit exacerbated pulmonary inflammation and dramatically increased endotoxin induced serum TNF levels.

Detailed description

Mice homozygous for a Tnfrsf1b^tm1Imx targeted mutation (formerly Tnfr2^tm1Imx, p75 deficient) are viable and fertile. T cells from homozygous mutant mice display defects in activation induced cell death in vitro and dramatically elevated levels of circulating Tnf following systemic challenge with LPS. Pulmonary neutrophil influx is exacerbated in p75 deficient mice in a model of hypersensitivity pneumonitis.

Development

The p75 mutation was made by homologous recombination in the 129/SvEv derived AB1 ES cell line using a gene replacement vector in which a 1 kb Kas1-BamH1 fragment was replaced with a PGKneo cassette. The resulting null mutation results in a deletion of amino acids 3 - 26. This strain was generated on a 129/SvEv background and is currently at N5 on a C57BL/6 background.

Allele

Symbol: Tnfrsf1b^tm1Imx
Name: targeted mutation 1, Immunex Research and Development Corporation
MGI accession ID: MGI:1860087
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Tnfrsf1b
Marker name: tumor necrosis factor receptor superfamily, member 1b
Chromosome: 4
Strain of origin: 129S7/SvEvBrd-Hprt⁺
Molecular note: A PGK-neomycin resistance cassette replaced 1kb of sequence encoding amino acids 3 - 26. Lack of gene product was shown functionally.