These Tnfrsf1b knock-out mice exhibit exacerbated pulmonary inflammation and dramatically increased endotoxin induced serum TNF levels.
Dr. Jacques Peschon, Amgen
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Tnfrsf1b | tumor necrosis factor receptor superfamily, member 1b |
Mice homozygous for a Tnfrsf1btm1Imx targeted mutation (formerly Tnfr2tm1Imx, p75 deficient) are viable and fertile. T cells from homozygous mutant mice display defects in activation induced cell death in vitro and dramatically elevated levels of circulating Tnf following systemic challenge with LPS. Pulmonary neutrophil influx is exacerbated in p75 deficient mice in a model of hypersensitivity pneumonitis.
The p75 mutation was made by homologous recombination in the 129/SvEv derived AB1 ES cell line using a gene replacement vector in which a 1 kb Kas1-BamH1 fragment was replaced with a PGKneo cassette. The resulting null mutation results in a deletion of amino acids 3 - 26. This strain was generated on a 129/SvEv background and is currently at N5 on a C57BL/6 background.
Allele Name | targeted mutation 1, Immunex Research and Development Corporation |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | p75-; p75 KO; TNFbeta ko; TNFR2-; TNFRII-; Tnfrsf1btm1Imx |
Gene Symbol and Name | Tnfrsf1b, tumor necrosis factor receptor superfamily, member 1b |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 4 |
Molecular Note | A PGK-neomycin resistance cassette replaced 1kb of sequence encoding amino acids 3 - 26. Lack of gene product was shown functionally. |
Mutations Made By | Dr. Jacques Peschon, Amgen |
When using the p75- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003246 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Tnfrsf1b<tm1Imx>, 1 pair minimum |
Frozen Mouse Embryo | B6.129S7-Tnfrsf1b<tm1Imx>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Tnfrsf1b<tm1Imx>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Tnfrsf1b<tm1Imx>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129S7-Tnfrsf1b<tm1Imx>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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