Mice homozygous for both targeted mutations lack both interlekin-1 beta type 1 receptor (IL1R1) and tumor necrosis factor alpha p55 (type 1) receptor (TNFR1). They exhibit reduced inflammatory responses and delayed-type hypersensitivity response. They are also susceptible to Listeria monocytogenes infection and have defects in Peyer's patch development, splenic architecture, formation of germinal centers and liver regeneration.
Dr. Jacques Peschon, Amgen
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Tnfrsf1a | tumor necrosis factor receptor superfamily, member 1a |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Il1r1 | interleukin 1 receptor, type I |
Mice homozygous for both targeted mutations (called TNFRp55(-/-)-IL-1RI(-/-), TNFR1/IL1R1 or IL-1R1/TNFR1 KO) are viable and fertile, lacking both interlekin-1 beta type 1 receptor (IL1R1) and tumor necrosis factor alpha p55 (type 1) receptor (TNFR1). Double homozygotes exhibit characteristics of both the single "knockouts" including reduced inflammatory responses, reduced delayed-type hypersensitivity response, and remain highly susceptible to infection by Listeria monocytogenes. Homozygous mutant mice also have defects in Peyer's patch development, splenic architecture, formation of germinal centers and liver regeneration. Furthermore, IL-1R1/TNFR1 KO mice exhibit alterations in rapid eye movement sleep (REMS) and non-rapid eye movement sleep (NREMS) (a sleep phenotype different from that observed for mice singly homozygous for one or the other of these cytokine receptors), as well as differences in NREMS and REMS following sleep deprivation compared to control mice.
Allele Name | targeted mutation 1, Immunex Research and Development Corporation |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | p55-; p55 KO; Preschon Tnfrsf1a-; TNF-alpha-R1; TNFR-; TNFR1-; TNFRI-; Tnfrsf1atm1Imx |
Gene Symbol and Name | Tnfrsf1a, tumor necrosis factor receptor superfamily, member 1a |
Gene Synonym(s) | |
Strain of Origin | C57BL/6 |
Chromosome | 6 |
Molecular Note | A PGK-neomycin resistance cassette replaced 4kb of sequence, including exons 2 to 5 (amino acids 30 - 184). Lack of gene product was shown functionally. |
Mutations Made By | Dr. Jacques Peschon, Amgen |
Allele Name | targeted mutation 1, Immunex Research and Development Corporation |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | IL-1R-; Il1rtm1Imx; IL-1R1-; Il1r1tm1jmx; IL-1RI KO; IL-R1-; RIko |
Gene Symbol and Name | Il1r1, interleukin 1 receptor, type I |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 1 |
Molecular Note | A neomycin resistance cassette replaced two exons of the gene, which encode amino acids 4 - 225 of the mature protein. |
Mutations Made By | Dr. Jacques Peschon, Amgen |
The strain is maintained by mating mice homozygous for both targeted mutations together (HOM HOM x HOM HOM). Expected coat color from breeding is White Bellied Agouti or Black.
When using the p55-;IL-1R- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003244 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Tnfrsf1a<tm1Imx>, Heterozygous for Il1r1<tm1Imx> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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