B6;129S-Tnfrsf1a^tm1Imx Il1r1^tm1Imx/J

Stock number: 003244
Product name: p55^-;IL-1R^-
Research areas: Apoptosis Research, Cancer Research, Immunology, Inflammation and Autoimmunity Research

Description

Mice homozygous for both targeted mutations lack both interlekin-1 beta type 1 receptor (IL1R1) and tumor necrosis factor alpha p55 (type 1) receptor (TNFR1). They exhibit reduced inflammatory responses and delayed-type hypersensitivity response. They are also susceptible to Listeria monocytogenes infection and have defects in Peyer's patch development, splenic architecture, formation of germinal centers and liver regeneration.

Detailed description

Mice homozygous for both targeted mutations (called TNFRp55(-/-)-IL-1RI(-/-), TNFR1/IL1R1 or IL-1R1/TNFR1 KO) are viable and fertile, lacking both interlekin-1 beta type 1 receptor (IL1R1) and tumor necrosis factor alpha p55 (type 1) receptor (TNFR1). Double homozygotes exhibit characteristics of both the single "knockouts" including reduced inflammatory responses, reduced delayed-type hypersensitivity response, and remain highly susceptible to infection by Listeria monocytogenes. Homozygous mutant mice also have defects in Peyer's patch development, splenic architecture, formation of germinal centers and liver regeneration. Furthermore, IL-1R1/TNFR1 KO mice exhibit alterations in rapid eye movement sleep (REMS) and non-rapid eye movement sleep (NREMS) (a sleep phenotype different from that observed for mice singly homozygous for one or the other of these cytokine receptors), as well as differences in NREMS and REMS following sleep deprivation compared to control mice.

Allele

Symbol: Il1r1^tm1Imx
Name: targeted mutation 1, Immunex Research and Development Corporation
MGI accession ID: MGI:1861112
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il1r1
Marker name: interleukin 1 receptor, type I
Chromosome: 1
Strain of origin: 129S7/SvEvBrd-Hprt⁺
Molecular note: A neomycin resistance cassette replaced two exons of the gene, which encode amino acids 4 - 225 of the mature protein.

Allele

Symbol: Tnfrsf1a^tm1Imx
Name: targeted mutation 1, Immunex Research and Development Corporation
MGI accession ID: MGI:1857468
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Tnfrsf1a
Marker name: tumor necrosis factor receptor superfamily, member 1a
Chromosome: 6
Strain of origin: C57BL/6
Molecular note: A PGK-neomycin resistance cassette replaced 4kb of sequence, including exons 2 to 5 (amino acids 30 - 184). Lack of gene product was shown functionally.