These mPCC mice express a transgene containing pigeon cytochrome c engineered for lymphoid tissue expression as a type II plasma membrane protein.
Dr. Stephen Hedrick, University of California at San Diego
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Allele Type | Gene Symbol | Gene Name |
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Not Applicable | H2 | histocompatibility-2, MHC |
PCC transgenic mice are viable and fertile. Pigeon cytochrome c was engineered for expression in lymphoid tissues of transgenic mice in two forms: one in which it is expressed as a type II plasma membrane protein (mPCC), and a second in which it was targeted to the mitochondria after cytoplasmic synthesis (ePCC). This strain carries the mPCC construct. (For mice carrying the ePCC construct, see strain 003221.) PCC is expressed in thymus, spleen and LPS stimulated B cells in both strains of mice. mRNA expression levels are higher in mPCC mice than in ePCC mice. However, tolerance is induced in the thymus of both strains, regardless of antigen compartmentation.
Expressed Gene | mPCC, pigeon cytochrome c - plasma membrane, pigeon |
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Site of Expression | |
Site of Expression |
Allele Name | transgene insertion 2939, Stephan Oehen |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | mPCC |
Gene Symbol and Name | Tg(H2KmPCC)2939Stoe, transgene insertion 2939, Stephan Oehen |
Gene Synonym(s) | |
Promoter | H2-K, histocompatibility 2, K region, mouse, laboratory |
Expressed Gene | mPCC, pigeon cytochrome c - plasma membrane, pigeon |
Strain of Origin | C57BL/6 x SJL |
Chromosome | UN |
Molecular Note | Pigeon cytochrome c was engineered for expression in lymphoid tissues of transgenic mice in two forms. The form of cytochrome c in this construct is expressed as a type II plasma membrane protein (mPCC) by replacing the NH2-terminal mitochondrial targeting sequence with the influenzaneuraminidase type II signal anchor. mRNA expression levels are higher in mice carrying this transgene than in mice carrying Tg(H2KePCC)2403. PCC was expressed in thymus, spleen and LPS stimulated B cells as determined by Northern blot. |
Mutations Made By | Dr. Stephan Oehen, Cytos Biotechnology AG |
Homozygotes are not viable.
When using the mPCC mouse strain in a publication, please cite the originating article(s) and include JAX stock #003240 in your Materials and Methods section.
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