cellular phenotype

• decreased T cell proliferation
  • T cells proliferate poorly in response to keyhole limpet hemocyanin stimulation compared to wild-type
  (MGI Ref ID J:31601)

immune system phenotype

• abnormal CD4-positive, alpha-beta T cell physiology
  • when day 16 fetal liver cells are transferred into wild-type mice, CD4+ T cell development is normal and when wild-type fetal cells are transferred into homozygotes, CD4+ T cells fail to mature
  (MGI Ref ID J:31601)
  • CD4+ T cell responses are significantly reduced to allogeneic stimulation
  (MGI Ref ID J:31601)

• abnormal cytokine secretion
  • spleen and lymph node cells produced no measurable cytokines at 8 weeks after infection
  (MGI Ref ID J:64283)

• abnormal level of surface class II molecules
  • bone marrow-derived dendritic cells do not express MHC class II molecules
  (MGI Ref ID J:96421)
  • MHC class II molecules are not detected in organs of mutants, except for interdigitating reticular cells where expression is not induced by interferon-gamma injection
  (MGI Ref ID J:31601)

• abnormal MHC II cell surface expression on macrophages
  • macrophages do not express MHC II cell surface expression on interferon-gamma stimulation
  (MGI Ref ID J:31601)

• abnormal B cell physiology
  • the expression of IL-10 after stimulation with LPS of IL-4 for 3 days is enhanced in mutant B cells by about 2-3 fold
  (MGI Ref ID J:96421)

• abnormal T cell physiology
  (MGI Ref ID J:31601)

• increased CD8-positive, alpha-beta T cell number
  • in spleen cell, a slight increase in the number of CD8+ T cells compared to wild-type is seen pre- and post-infection
  (MGI Ref ID J:64283)

• abnormal susceptibility to infection
  (MGI Ref ID J:64283)

• abnormal popliteal lymph node morphology
  • total numbers of cells in lymph nodes of null increased only slightly after infection with L. amazonensis compared with an 8-fold increase in size of wild-type
  (MGI Ref ID J:64283)

• decreased CD4-positive, alpha beta T cell number
  • mice have reduced numbers of mature CD4+ T cells in the periphery
  (MGI Ref ID J:31601)

• decreased susceptibility to parasitic infection
  • after infection with L. amazonensis, infected cells contained tightly packed organisms with few parasitophorous vacuole visible whereas infected macrophages in wild-type mice contained only a few organisms which are preferentially located at the edge of parasitophorous vacuoles
  (MGI Ref ID J:64283)
  • after infection with L. Amazonensis, mice show no lesion development up to 11 weeks post infection, reduced cellular infiltration, lower parasite load and few cells in the popliteal lymph nodes draining the foot
  (MGI Ref ID J:64283)
  • arasitophorous vacuoles
  (MGI Ref ID J:64283)

• decreased T cell proliferation
  • T cells proliferate poorly in response to keyhole limpet hemocyanin stimulation compared to wild-type
  (MGI Ref ID J:31601)

• increased CD4-positive, alpha beta T cell number
  • in spleen cells, there is a 4.5-fold increase of CD4+CD69^high cells in mutants at 8 weeks after parasite infection
  (MGI Ref ID J:64283)

• abnormal T cell differentiation
  (MGI Ref ID J:64283)

• increased susceptibility to parasitic infection
  • lesions become significantly larger with higher parasitic burdens 16 weeks after infection with L. major, compared to eventual lesion resolution in wild-type mice
  (MGI Ref ID J:64283)

• abnormal dendritic cell physiology
  • Bone marrow-derived mutant dendritic cells in culture differentiate in response to GM-CSF despite absence of MHC class II molecules; however, when stimulated with LPS of CpG display about a 2-fold increase in IL-10 expression compared to wild-type
  (MGI Ref ID J:96421)

• abnormal lymphocyte physiology
  (MGI Ref ID J:96421)

hematopoietic system phenotype

• abnormal T cell physiology
  (MGI Ref ID J:31601)

• increased CD8-positive, alpha-beta T cell number
  • in spleen cell, a slight increase in the number of CD8+ T cells compared to wild-type is seen pre- and post-infection
  (MGI Ref ID J:64283)

• abnormal B cell physiology
  • the expression of IL-10 after stimulation with LPS of IL-4 for 3 days is enhanced in mutant B cells by about 2-3 fold
  (MGI Ref ID J:96421)

• abnormal T cell differentiation
  (MGI Ref ID J:64283)

• increased CD4-positive, alpha beta T cell number
  • in spleen cells, there is a 4.5-fold increase of CD4+CD69^high cells in mutants at 8 weeks after parasite infection
  (MGI Ref ID J:64283)

• decreased CD4-positive, alpha beta T cell number
  • mice have reduced numbers of mature CD4+ T cells in the periphery
  (MGI Ref ID J:31601)

• decreased T cell proliferation
  • T cells proliferate poorly in response to keyhole limpet hemocyanin stimulation compared to wild-type
  (MGI Ref ID J:31601)

• abnormal CD4-positive, alpha-beta T cell physiology
  • CD4+ T cell responses are significantly reduced to allogeneic stimulation
  (MGI Ref ID J:31601)
  • when day 16 fetal liver cells are transferred into wild-type mice, CD4+ T cell development is normal and when wild-type fetal cells are transferred into homozygotes, CD4+ T cells fail to mature
  (MGI Ref ID J:31601)

• abnormal lymphocyte physiology
  (MGI Ref ID J:96421)

• abnormal MHC II cell surface expression on macrophages
  • macrophages do not express MHC II cell surface expression on interferon-gamma stimulation
  (MGI Ref ID J:31601)

immune system phenotype

• abnormal level of surface class II molecules
  • class II expression is absent on CD11c⁺ dendritic cells
  (MGI Ref ID J:67941)
  • class II surface expression in the cortex and medulla of the thymus is very patchy
  (MGI Ref ID J:67941)
  • unactivated or activated B cells do not express class II on the surface
  (MGI Ref ID J:67941)