These alpha7 nicotinic acetylcholine receptor knockout mice may be useful in studying the cholinergic anti-inflammatory pathway; including inflammatory neurotoxicity in stroke, myocardial infarction, sepsis and Alzheimer's disease, as well as neuropsychiatric diseases such as schizophrenia.
Dr. Arthur Beaudet, Baylor College of Medicine
Genetic Background | Generation |
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N10F13
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Chrna7 | cholinergic receptor, nicotinic, alpha polypeptide 7 |
Starting at:
$255.00 Domestic price for female 4-week |
510.00 Domestic price for breeder pair |
Mice homozygous for the alpha7 nicotinic acetylcholine receptor null allele (α7 nAChR- or α7-) are viable and fertile. Neuropathological and histochemical assessment of brains from α7 nAChR knockout mice reveal no abnormalities. High-affinity nicotine binding sites are present but there is an absence of high-affinity [I-125] alpha-bungarotoxin sites. Homozygotes lack rapidly desensitizing, methyllycaconitine-sensitive, nicotinic currents that are present in hippocampal neurons.
A null mutation of the alpha7 subunit was prepared by deleting the last three exons (8-10) of the Chrna7 gene. Mice deficient in the alpha 7 subunit were generated by introducing a 7 kb deletion into ES cells. The strain originated on a mixed 129/SvEv and C57BL/6 background. The donating investigator backcrossed these mice to C57BL/6 for approximately eight generations ("N8F8") prior to sending to The Jackson Laboratory.
Allele Name | targeted mutation 1, Baylor College of Medicine |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Acralpha7 -; alpha7; alpha7 NNR -; Chrna7Baytm1 |
Gene Symbol and Name | Chrna7, cholinergic receptor, nicotinic, alpha polypeptide 7 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 7 |
Molecular Note | A 7 kb genomic fragment containing exons 8-10 was replaced with a neomycin selection cassette. The deleted sequences encode the second through the fourth transmembrane domains and the cytoplasmic loop. Northern blot analysis on brain samples derived from homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot analysis on brain extracts derived from homozygous mice also confirmed that no detectable protein was made from this allele. |
Mutations Made By | Dr. Arthur Beaudet, Baylor College of Medicine |
When maintaining a live colony, homozygous mice may be bred to heterozygous mice. Although homozygotes are viable and fertile, the donating investigator reports that breeding is better using heterozygous breeders, and they have observed reduced fertility in homozygotes. Heterozygotes produce small litters. Expected coat color from breeding is Black.
When using the Acrα7- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003232 in your Materials and Methods section.
Service/Product | Description | Price |
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Heterozygous for Chrna7<tm1Bay> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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